B-cell responses result in clonal expansion, and can occur in a variety of tissues. To define how B-cell clones are distributed in the body, we sequenced 933,427 B-cell clonal lineages and mapped them to eight different anatomic compartments in six human organ donors. We show that large B-cell clones partition into two broad networks-one spans the blood, bone marrow, spleen and lung, while the other is restricted to tissues within the gastrointestinal (GI) tract (jejunum, ileum and colon). Notably, GI tract clones display extensive sharing of sequence variants among different portions of the tract and have higher frequencies of somatic hypermutation, suggesting extensive and serial rounds of clonal expansion and selection. Our findings provide an anatomic atlas of B-cell clonal lineages, their properties and tissue connections. This resource serves as a foundation for studies of tissue-based immunity, including vaccine responses, infections, autoimmunity and cancer.

中文翻译：

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人体内 B 细胞克隆分布图谱。


B 细胞反应导致克隆扩增，并且可以发生在多种组织中。为了定义 B 细胞克隆在体内的分布方式，我们对 933,427 个 B 细胞克隆谱系进行了测序，并将它们映射到 6 个人体器官捐献者的 8 个不同的解剖区室中。我们发现，大型 B 细胞克隆分为两个广泛的网络 - 一个跨越血液、骨髓、脾和肺，而另一个仅限于胃肠道 (GI) 内的组织（空肠、回肠和结肠）。值得注意的是，胃肠道克隆在消化道的不同部分之间显示出广泛共享的序列变异，并且具有更高的体细胞超突变频率，这表明存在广泛且连续的克隆扩增和选择。我们的研究结果提供了 B 细胞克隆谱系、其特性和组织连接的解剖图谱。该资源是基于组织的免疫研究的基础，包括疫苗反应、感染、自身免疫和癌症。