Photo-induced processes have high potential in in situ patterning and controlling living cells, whose developments are introduced and recent progresses by utilizing femtosecond laser are described. Photochemical and photothermal surface modification performed by conventional light and nanosecond laser irradiation is summarized and their applicability is considered. Femtosecond laser ablation has superior features due to its photomechanical mechanism, which is confirmed by ultrafast spectroscopy and imaging of a model film under laser ablation. Femtosecond laser ablation of physiological solutions generates shockwave and cavitation bubbles, which is employed for patterning and manipulating living cells. Femtosecond laser ablation fabricating cytophobic and cytophilic domains enable us to form living cell patterns and to study cell migration and cell–cell interaction. Finally summary and perspective are presented.

光诱导过程在原位具有很高的潜力图案化和控制活细胞，介绍了其发展，并介绍了利用飞秒激光的最新进展。总结了通过常规光和纳秒激光辐照进行的光化学和光热表面改性，并考虑了它们的适用性。飞秒激光烧蚀由于其光机械机理而具有卓越的功能，这一点已通过超快光谱学和在激光烧蚀下对模型膜的成像得到证实。飞秒激光烧蚀生理溶液会产生冲击波和空化气泡，这些气泡用于图案化和操纵活细胞。飞秒激光烧蚀制造了具有疏水性和亲细胞性的区域，使我们能够形成活细胞模式并研究细胞迁移和细胞间相互作用。