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Engineering and In Vivo Applications of Riboswitches
Annual Review of Biochemistry ( IF 12.1 ) Pub Date : 2017-06-27 00:00:00 , DOI: 10.1146/annurev-biochem-060815-014628
Zachary F. Hallberg 1 , Yichi Su 1 , Rebekah Z. Kitto 1 , Ming C. Hammond 1, 2
Affiliation  

Riboswitches are common gene regulatory units mostly found in bacteria that are capable of altering gene expression in response to a small molecule. These structured RNA elements consist of two modular subunits: an aptamer domain that binds with high specificity and affinity to a target ligand and an expression platform that transduces ligand binding to a gene expression output. Significant progress has been made in engineering novel aptamer domains for new small molecule inducers of gene expression. Modified expression platforms have also been optimized to function when fused with both natural and synthetic aptamer domains. As this field expands, the use of these privileged scaffolds has permitted the development of tools such as RNA-based fluorescent biosensors. In this review, we summarize the methods that have been developed to engineer new riboswitches and highlight applications of natural and synthetic riboswitches in enzyme and strain engineering, in controlling gene expression and cellular physiology, and in real-time imaging of cellular metabolites and signals.

中文翻译:


核糖开关的工程和体内应用

核糖开关是常见的基因调节单位,主要存在于细菌中,能够响应小分子改变基因表达。这些结构化的RNA元件由两个模块性亚基组成:与目标配体具有高特异性和亲和力的适体结构域和将配体与基因表达输出结合的表达平台。在用于基因表达的新小分子诱导物的工程新型适体结构域中已经取得了重大进展。修饰的表达平台还经过优化,可在与天然和合成适体结构域融合时发挥作用。随着该领域的扩展,这些特权支架的使用已允许开发诸如基于RNA的荧光生物传感器之类的工具。在这篇评论中,

更新日期:2017-06-27
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