Porous silicon nanoneedles can interface with cells and tissues with minimal perturbation for high-throughput intracellular delivery and biosensing. Typically, nanoneedle devices are rigid, flat, and opaque, which limits their use for topical applications in the clinic. We have developed a robust, rapid, and precise substrate transfer approach to incorporate nanoneedles within diverse substrates of arbitrary composition, flexibility, curvature, transparency, and biodegradability. With this approach, we integrated nanoneedles on medically relevant elastomers, hydrogels, plastics, medical bandages, catheter tubes, and contact lenses. The integration retains the mechanical properties and transfection efficiency of the nanoneedles. Transparent devices enable the live monitoring of cell–nanoneedle interactions. Flexible devices interface with tissues for efficient, uniform, and sustained topical delivery of nucleic acids ex vivo and in vivo. The versatility of this approach highlights the opportunity to integrate nanoneedles within existing medical devices to develop advanced platforms for topical delivery and biosensing.

中文翻译：

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将多孔硅纳米针集成到医疗器械中用于核酸纳米注射

多孔硅纳米针可以以最小的扰动与细胞和组织接触，以实现高通量细胞内递送和生物传感。通常，纳米针装置是刚性的、扁平的且不透明的，这限制了它们在临床中的局部应用。我们开发了一种稳健、快速且精确的基材转移方法，将纳米针整合到任意成分、灵活性、曲率、透明度和生物可降解性的各种基材中。通过这种方法，我们将纳米针集成到医学相关的弹​​性体、水凝胶、塑料、医用绷带、导管和隐形眼镜上。这种集成保留了纳米针的机械特性和转染效率。透明设备能够实时监测细胞与纳米针的相互作用。灵活的装置与组织连接，可在离体和体内有效、均匀和持续地局部输送核酸。这种方法的多功能性凸显了将纳米针集成到现有医疗设备中以开发用于局部输送和生物传感的先进平台的机会。