In this work, miRNA-10b in the glioblastoma (GBM) tumor tissues has been detected by a novel electrochemiluminescence (ECL) biosensor. Firstly, a new kind of bright luminescent ZnGeO:Mn NPs were prepared as ECL nanoprobe, which possessed high fluorescence quantum yield and ECL quantum efficiency. Secondly, TiC MXene hydrogel (MXG) have been developed as the sensing interface. The MXG retained the inherent biocompatibility and mechanical features of hydrogel. Furthermore, the uniform distribution of metallic TiC MXene in the hydrogel microstructure provided the good conductivity and multiple binding sites for biomolecules. MXene also can promote the separation of the electrons and holes to accelerate the electron-transfer rate and improve ECL efficiency. Due to these synergistic effects, the screen printed electrode was successfully modified with MXG as sensing platform to enhance the ECL intensity of ZnGeO:Mn NP, which greatly improved the detection efficiency and facilitated the high-throughput analysis. Finally, the toehold mediated strand displacement (TMSD) strategy was employed with then biosensor to detect miRNA-10b with the range of 10 fM to 1 nM. The limit of detection was 5 fM. This ECL biosensor has been used to analyze miRNA-10b expression in GBM tumor tissues, which possessed the great potential value for clinical diagnosis.

中文翻译：

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用于胶质母细胞瘤诊断的基于 ECL 的明亮发光 Zn2GeO4:Mn NP/MXene 水凝胶生物传感器

在这项工作中，胶质母细胞瘤 (GBM) 肿瘤组织中的 miRNA-10b 通过新型电化学发光 (ECL) 生物传感器进行了检测。首先，制备了一种新型明亮发光的ZnGeO:Mn NPs作为ECL纳米探针，其具有高荧光量子产率和ECL量子效率。其次，TiC MXene 水凝胶（MXG）已被开发作为传感界面。 MXG 保留了水凝胶固有的生物相容性和机械特性。此外，金属TiC MXene在水凝胶微结构中的均匀分布为生物分子提供了良好的导电性和多个结合位点。 MXene还可以促进电子和空穴的分离，从而加快电子转移速率并提高ECL效率。由于这些协同效应，以MXG为传感平台成功修饰了丝网印刷电极，增强了ZnGeO:Mn NP的ECL强度，大大提高了检测效率，有利于高通量分析。最后，采用立足点介导链置换（TMSD）策略与生物传感器检测10 fM至1 nM范围内的miRNA-10b。检测限为 5 fM。该ECL生物传感器已用于分析GBM肿瘤组织中miRNA-10b的表达，具有巨大的临床诊断潜在价值。