HSO is an important reactive sulfur species that maintains the normal physiological activities of living organisms and participates in a variety of redox homeostatic processes. It has been found that changes in HSO levels is closely related to the heat stroke phenomenon of the organism. Heat stroke causes damage to normal cells, which in turn causes damage to the body and even death. It is crucial to accurately monitor and track the physiological behavior of HSO during heat stroke. Herein, a ratiometric multifunctional fluorescent probe with dual-targeting ability to rapidly and precisely recognize HSO being constructed based on the FRET mechanism. has extra Large Stokes shift (216 nm), very high sensitivity (DL = 12.2 nM), fast response time and good specificity. When undergoes Michael addition with HSO, the fluorescence emission peak was blue-shifted from 616 nm to 472 nm, and a clear ratiometric signal appeared. The interaction between lysosomes and mitochondria in maintaining cellular homeostasis was investigated by the dual-targeting ability of the probe using HSO as a mediator. achieved successful targeting and real-time monitoring of exogenous and endogenous HSO in the cells. More importantly, imaging experiments in heat stroke mice revealed high HSO expression in intestinal tissues. This provides new ideas and research tools for early prevention of heat stroke-induced diseases such as intestinal injuries. In addition, the semi-quantitative monitoring experiments for paper-based visualization of HSO make the probe promising for the design of portable detectors.

中文翻译：

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具有双靶向能力的比率荧光探针，用于热激成像

HSO是一种重要的活性硫物种，维持生物体的正常生理活动并参与多种氧化还原稳态过程。研究发现HSO水平的变化与机体的中暑现象密切相关。中暑会对正常细胞造成损害，进而导致身体受损，甚至死亡。准确监测和跟踪热射病期间 HSO 的生理行为至关重要。在此，基于FRET机制构建了一种具有双靶向能力的比例式多功能荧光探针，能够快速准确地识别HSO。具有超大斯托克斯位移 (216 nm)、非常高的灵敏度 (DL = 12.2 nM)、快速的响应时间和良好的特异性。当与HSO进行迈克尔加成时，荧光发射峰从616 nm蓝移至472 nm，并出现清晰的比率信号。通过使用 HSO 作为介质的探针的双靶向能力，研究了溶酶体和线粒体在维持细胞稳态中的相互作用。成功靶向并实时监测细胞中的外源性和内源性 HSO。更重要的是，中暑小鼠的成像实验揭示了肠道组织中 HSO 的高表达。这为早期预防肠道损伤等中暑诱发疾病提供了新思路和研究工具。此外，基于纸张的 HSO 可视化半定量监测实验使该探针有望用于便携式探测器的设计。