To enhance food safety, the need for swift and precise detection of , a bacterium prevalent in various environments, including soil and food products, is paramount. This study presents an innovative and cost-effective bioassay designed to specifically identify the foodborne pathogen, , utilizing a colorimetric signal approach. The biosensor, featuring a 3D-printed architecture, incorporates a casein-based liquid-proof gelatine film, selectively liquefying in response to the caseinolytic/proteolytic activity of external enzymes from the pathogen. As the sample liquefies, it progresses through a color layer, causing the migration of dye to an absorbent layer, resulting in a distinct positive signal. This bioassay exhibits exceptional sensitivity, detecting concentrations as low as 1 CFU/mL within a 9.3-h assay duration. Notably, this cost-efficient bioassay outperforms conventional methods in terms of efficacy and cost-effectiveness, offering a straightforward solution for promptly detecting in food samples.

中文翻译：

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提高食品安全：用于食品中地衣芽孢杆菌检测的低成本生物传感器

为了提高食品安全性，快速、精确地检测 细菌是一种普遍存在于各种环境（包括土壤和食品）中的细菌，这一点至关重要。这项研究提出了一种创新且具有成本效益的生物测定法，旨在利用比色信号方法专门识别食源性病原体。该生物传感器采用 3D 打印架构，采用酪蛋白基防液明胶膜，可选择性液化以响应病原体外部酶的酪蛋白分解/蛋白水解活性。当样品液化时，它会穿过颜色层，导致染料迁移到吸收层，从而产生明显的阳性信号。该生物测定具有卓越的灵敏度，可在 9.3 小时的测定时间内检测低至 1 CFU/mL 的浓度。值得注意的是，这种经济高效的生物测定在功效和成本效益方面优于传统方法，为食品样品中的快速检测提供了一种简单的解决方案。