Due to the common contamination of multiple mycotoxins in food, which results in stronger toxicity, it is particularly important to simultaneously test for various mycotoxins for the protection of human health. In this study, a disposable immunosensor array with low-cost was designed and fabricated using cellulose paper, polydimethylsiloxane (PDMS), and semiconducting single-walled carbon nanotubes (s-SWCNTs), which was modified with specific antibodies for mycotoxins AFB1 and FB1 detection. The strategy for fabricating the immunosensor array with two individual channels involved a two-step protocol starting with the form of two kinds of carbon films by depositing single-wall carbon nanotubes (SWCNTs) and s-SWCNTs on the cellulose paper as the conductive wire and sensing element, followed by the assembly of chemiresistive biosensor with SWCNTs strip as the wire and s-SWCNTs as the sensing element. After immobilizing AFB1-bovine serum albumin (AFB1-BSA) and FB1-bovine serum albumin (FB1-BSA) separately on the different sensing regions, the formation of mycotoxin-BSA-antibody immunocomplexes transfers to electrochemical signal, which would change with the different concentrations of free mycotoxins. Under optimal conditions, the immunosensor array achieved a limit of detection (LOD) of 0.46 pg/mL for AFB1 and 0.34 pg/mL for FB1 within a wide dynamic range from 1 pg/mL to 20 ng/mL. Furthermore, the AFB1 and FB1 spiked in the ground corn and wheat extracts were detected with satisfactory recoveries, demonstrating the excellent practicality of this established method for simultaneous detection of mycotoxins.

中文翻译：

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使用纤维素纸组装化学电阻生物传感器的一次性免疫传感器阵列，用于同时监测霉菌毒素 AFB1 和 FB1

由于食品中多种霉菌毒素共同污染，毒性较强，因此同时检测多种霉菌毒素对于保护人类健康显得尤为重要。在这项研究中，使用纤维素纸、聚二甲基硅氧烷（PDMS）和半导体单壁碳纳米管（s-SWCNT）设计和制造了一种低成本的一次性免疫传感器阵列，并用特异性抗体进行修饰，用于霉菌毒素AFB1和FB1检测。制造具有两个单独通道的免疫传感器阵列的策略涉及两步方案，首先通过在纤维素纸上沉积单壁碳纳米管（SWCNT）和 s-SWCNT 作为导线，形成两种碳膜，然后传感元件，然后以单壁碳纳米管条作为导线，以单壁碳纳米管作为传感元件组装化学电阻生物传感器。将AFB1-牛血清白蛋白（AFB1-BSA）和FB1-牛血清白蛋白（FB1-BSA）分别固定在不同的传感区域后，霉菌毒素-BSA-抗体免疫复合物的形成转化为电化学信号，该信号会随着不同的传感区域而变化。游离霉菌毒素的浓度。在最佳条件下，免疫传感器阵列在 1 pg/mL 至 20 ng/mL 的宽动态范围内，AFB1 的检测限 (LOD) 为 0.46 pg/mL，FB1 的检测限为 0.34 pg/mL。此外，在磨碎的玉米和小麦提取物中添加的AFB1和FB1均得到了令人满意的回收率，证明了该建立的霉菌毒素同步检测方法具有良好的实用性。