In this study, peptide‐based self‐assembled nanosheets with a thickness of approximately 1 nm were prepared using a hierarchical covalent physical fabrication strategy. The covalent alternating polymerization of helical peptide E3 with an azobenzene (AZO) structure yielded copolymers CoP(E3–AZO), which physically self‐assembled into ultrathin nanosheets in an unanticipated two‐dimensional horizontal monolayer arrangement. This special monolayer arrangement enabled the thickness of the nanosheets to be equal to the cross‐sectional diameter of a single linear copolymer, which is a rare phenomenon. Molecular dynamics simulations suggested that the synergistic effect of multiple molecular interactions drives the self‐assembly of CoP(E3–AZO) into nanosheets and that various methods, including phototreatment, pH adjustment, the addition of additives, and introduction of cosolvents, can alter the molecular interactions and modulate the self‐assembly of CoP(E3–AZO), yielding diverse nanostructures. Remarkably, the ultrathin nanosheets selectively inhibited cancer cells at certain concentrations.

中文翻译：

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水平单层组装制备的多响应肽基超薄纳米片

在这项研究中，使用分层共价物理制造策略制备了厚度约为 1 nm 的基于肽的自组装纳米片。螺旋肽 E3 与偶氮苯 (AZO) 结构的共价交替聚合产生了共聚物 CoP(E3-AZO)，它以意想不到的二维水平单层排列物理自组装成超薄纳米片。这种特殊的单层排列使得纳米片的厚度等于单一线性共聚物的横截面直径，这是一种罕见的现象。分子动力学模拟表明，多种分子相互作用的协同效应驱动CoP(E3-AZO)自组装成纳米片，并且各种方法，包括光处理、pH调节、添加添加剂和引入共溶剂，可以改变分子相互作用并调节 CoP(E3-AZO) 的自组装，产生不同的纳米结构。值得注意的是，超薄纳米片在一定浓度下选择性地抑制癌细胞。