As a new generation of economically important fruit, blackberry fruit is rich in nutrients and functional components. However, the ripe fruit is very low in firmness, soft, and perishable. Therefore, storage and preservation have become important factors restricting its industrial development. The overexpression vectors of and encoded endo-1,4-β-glucanase from blackberry were introduced into tomato to identify their functions at different ripening stages. The results showed that firmness of transgenic tomato fruit decreased with the development period. The structure of transgenic tomato collapsed earlier than that of wild-type tomato. The comparison showed that the contents of cellulose, hemicellulose, and pectin in transgenic tomato were significantly reduced compared with wild-type tomato. Pectin methylesterase and cellulase activities were significantly increased in transgenic compared with wild-type tomato. Transcriptome and qRT-PCR data showed that the expression levels of cell wall degrading enzyme genes and were significantly higher than those of the control. The genes of and regulated enzyme activity in fruit cell walls and influenced cell wall composition, thus regulating fruit firmness and improving fruit quality and storability. In addition, the and genes were higher expressed in than in transgenic fruits at the yellow fruit stage, which might be the reason why they reveal divergent regulation during fruit ripening stages.

中文翻译：

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黑莓的两个内切 1,4-β-葡聚糖酶基因家族成员揭示了果实成熟过程中的不同调控


黑莓果实作为新一代重要经济水果，含有丰富的营养成分和功能成分。然而，成熟的果实硬度很低，柔软，容易腐烂。因此，储存和保鲜已成为制约其产业发展的重要因素。将黑莓编码的内切 1,4-β-葡聚糖酶的过表达载体引入番茄中，以鉴定其在不同成熟阶段的功能。结果表明，转基因番茄果实的硬度随发育期的延长而降低。转基因番茄的结构比野生型番茄更早崩溃。比较表明，转基因番茄中纤维素、半纤维素、果胶的含量较野生型番茄显着降低。与野生型番茄相比，转基因番茄的果胶甲酯酶和纤维素酶活性显着增加。转录组和qRT-PCR数据显示，细胞壁降解酶基因的表达水平显着高于对照。和 基因调控果实细胞壁酶活性，影响细胞壁组成，从而调节果实硬度，提高果实品质和耐贮性。此外， 和 基因在黄色果实阶段的表达量高于转基因果实，这可能是它们在果实成熟阶段表现出不同调控的原因。