Hypochlorous acid (HOCl) plays important roles in many pathological and physiological processes. Herein we present a novel strategy to develop HOCl probe by one-step synthesis of rhodamine-thiophene dyad, rho-T. Unexpectedly, the thiophene moiety did not cause the fluorescence quenching of the xanthene moiety. Upon addition of HOCl, the ClO anion attacks the carbon 9 position of rho-T and chlorinate the xanthene ring, resulting in fluorescence and absorption quenching of rho-T. rho-T exhibits linear response with increasing concentration of HOCl (R = 0.9875) with high sensitivity (Limit of Detection (LOD) = 2.36 nM) and selectivity in a fast response time (10 s). Finally, rho-T was successfully employed to detect HOCl in living cells and mice.

中文翻译：

---

罗丹明-噻吩二元体的一步合成及其在体外和体内 HOCl 检测中的应用


次氯酸（HOCl）在许多病理和生理过程中发挥着重要作用。在此，我们提出了一种通过一步合成罗丹明-噻吩二元体 rho-T 来开发 HOCl 探针的新策略。出乎意料的是，噻吩部分并没有引起呫吨部分的荧光猝灭。添加 HOCl 后，ClO 阴离子攻击 rho-T 的碳 9 位并氯化呫吨环，导致 rho-T 的荧光和吸收猝灭。 rho-T 随着 HOCl 浓度的增加呈现线性响应 (R = 0.9875)，具有高灵敏度（检测限 (LOD) = 2.36 nM）和快速响应时间 (10 s) 的选择性。最后，rho-T 成功用于检测活细胞和小鼠中的 HOCl。