In this work, we report a new generation of single microbead bioassay that employs a single BaTiO₃ microbead as an optical booster for target biomarker enrichment and optical enhancement toward protein and nucleic acid analysis. The single BaTiO₃ microbead can not only concentrate the target molecules by nearly 10⁴-fold but also act as an optical booster to prominently enhance the target-induced fluorescence signal by the whispering gallery mode for improving the excitation efficiency and the microlens effect for promoting the signal collecting efficiency, respectively. Compared with using a conventional single microbead, this optical booster exhibits nearly 2 orders of magnitude higher sensitivity without the assistance of any signal amplification techniques or costly instruments. Moreover, this single microbead optical booster is capable of detecting different kinds of protein and nucleic acid biomarkers in a simple mix-and-read manner, holding great potential for early clinical diagnosis.

中文翻译：

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单个 BaTiO3 微珠光学增强器上的增强荧光信号实现高灵敏度生物传感


在这项工作中，我们报告了新一代单微珠生物测定，它采用单个 BaTiO ₃ 微珠作为目标生物标志物富集和蛋白质和核酸分析的光学增强的光学助推器。单个 BaTiO ₃ 微珠不仅可以将目标分子浓缩近 10 ⁴ 倍，而且可以作为光学助推器，显着增强回音壁的目标诱导荧光信号分别提高激发效率的模式和提高信号收集效率的微透镜效应。与使用传统的单个微珠相比，这种光学增强器的灵敏度提高了近 2 个数量级，而无需任何信号放大技术或昂贵的仪器的帮助。此外，这种单微珠光学增强器能够以简单的混合读取方式检测不同种类的蛋白质和核酸生物标志物，在早期临床诊断方面具有巨大的潜力。