A novel strategy combining ferulic acid and glucose was proposed to reduce β-lactoglobulin (BLG) allergenicity and investigate whether the reduction in allergenicity was associated with gut microbiome and serum metabolism. As a result, the multistructure of BLG changed, and the modified BLG decreased significantly the contents of IgE, IgG, IgG₁, and mMCP-1 in serum, improved the diversity and structural composition of gut microbiota, and increased the content of short-chain fatty acids (SCFAs) in allergic mice. Meanwhile, allergic mice induced by BLG affected arachidonic acid, tryptophan, and other metabolic pathways in serum, the modified BLG inhibited the production of metabolites in arachidonic acid metabolism pathway and significantly increased tryptophan metabolites, and this contribution helps in reducing BLG allergenicity. Overall, reduced allergenicity of BLG after ferulic acid was combined with glucose modification by regulating gut microbiota, the metabolic pathways of arachidonic acid and tryptophan. The results may offer new thoughts alleviating the allergy risk of allergenic proteins.

中文翻译：

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肠道微生物组-血清代谢揭示了阿魏酸与葡萄糖修饰的 β-乳球蛋白结合的致敏性


提出了一种结合阿魏酸和葡萄糖的新策略来降低β-乳球蛋白（BLG）过敏性，并研究过敏性的降低是否与肠道微生物组和血清代谢相关。结果，BLG的多结构发生改变，修饰后的BLG显着降低了血清中IgE、IgG、IgG ₁ 和mMCP-1的含量，改善了肠道菌群的多样性和结构组成，增加过敏小鼠的短链脂肪酸（SCFA）含量。同时，BLG诱导的过敏小鼠影响了血清中的花生四烯酸、色氨酸等代谢途径，修饰后的BLG抑制了花生四烯酸代谢途径中代谢物的产生，显着增加了色氨酸代谢物，有助于降低BLG的致敏性。总体而言，阿魏酸与葡萄糖修饰相结合，通过调节肠道微生物群、花生四烯酸和色氨酸的代谢途径，降低了 BLG 的过敏原性。研究结果可能为减轻过敏蛋白的过敏风险提供新的思路。