Hybrid genome-mining/¹⁵N-NMR was used to target compounds containing piperazate (Piz) residues, leading to the discovery of caveamides A (1) and B (2) from Streptomyces sp. strain BE230, isolated from New Rankin Cave (Missouri). Caveamides are highly dynamic molecules containing an unprecedented β-ketoamide polyketide fragment, two Piz residues, and a new N-methyl-cyclohexenylalanine residue. Caveamide B (2) exhibited nanomolar cytotoxicity against several cancer cell lines and nanomolar antimicrobial activity against MRSA and E. coli.

中文翻译：

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哌嗪引导下从穴放线菌中分离 Caveamides A 和 B（含环己基丙氨酸的非核糖体肽）

混合基因组挖掘/ ¹⁵ N-NMR 用于靶向含有哌嗪 (Piz) 残基的化合物，从而发现了来自链霉菌属的卡夫酰胺 A ( 1 ) 和 B ( 2 ) 。菌株BE230，分离自New Rankin Cave（密苏里州）。 Caveamides 是高度动态的分子，含有前所未有的 β-酮酰胺聚酮化合物片段、两个 Piz 残基和一个新的N-甲基-环己基丙氨酸残基。 Caveamide B ( 2 ) 对几种癌细胞系表现出纳摩尔级细胞毒性，对 MRSA 和大肠杆菌表现出纳摩尔级抗菌活性。