Aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) mediate the responses of adaptive metabolism to various xenobiotics. Here, we found that BoAhR and BoARNT are highly expressed in the midgut of Bradysia odoriphaga larvae. The expression of BoAhR and BoARNT was significantly increased after exposure to imidacloprid and phoxim. The knockdown of BoAhR and BoARNT significantly decreased the expression of CYP6SX1 and CYP3828A1 as well as P450 enzyme activity and caused a significant increase in the sensitivity of larvae to imidacloprid and phoxim. Exposure to β-naphthoflavone (BNF) significantly increased the expression of BoAhR, BoARNT, CYP6SX1, and CYP3828A1 as well as P450 activity and decreased larval sensitivity to imidacloprid and phoxim. Furthermore, CYP6SX1 and CYP3828A1 were significantly induced by imidacloprid and phoxim, and the silencing of these two genes significantly reduced larval tolerance to imidacloprid and phoxim. Taken together, the BoAhR/BoARNT pathway plays key roles in larval tolerance to imidacloprid and phoxim by regulating the expression of CYP6SX1 and CYP3828A1.

中文翻译：

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转录因子 AhR 和 ARNT 调控 CYP6SX1 和 CYP3828A1 参与昆虫解毒的臭苋菌表达

芳基碳氢化合物受体（AhR）和芳基碳氢化合物受体核转位子（ARNT）介导适应性代谢对各种外源物质的反应。在这里，我们发现BoAhR和BoARNT在臭苋幼虫的中肠中高表达。吡虫啉和辛硫磷暴露后BoAhR和BoARNT的表达显着增加。 BoAhR和BoARNT的敲低显着降低了CYP6SX1和CYP3828A1的表达以及P450酶活性，并导致幼虫对吡虫啉和辛硫磷的敏感性显着增加。暴露于 β-萘黄酮 (BNF) 显着增加了BoAhR、BoARNT、CYP6SX1和CYP3828A1的表达以及 P450 活性，并降低了幼虫对吡虫啉和辛硫磷的敏感性。此外，CYP6SX1和CYP3828A1受到吡虫啉和辛硫磷的显着诱导，这两个基因的沉默显着降低了幼虫对吡虫啉和辛硫磷的耐受性。综上所述，BoAhR / BoARNT 途径通过调节CYP6SX1和CYP3828A1的表达，在幼虫对吡虫啉和辛硫磷的耐受性中发挥关键作用。