Liposcelis bostrychophila, commonly known as booklouse, is an important stored-product pest worldwide. Studies have demonstrated that booklices have developed resistance to several insecticides. In this study, an integument esterase gene, LbEST-inte4, with upregulated expression, was characterized in L. bostrychophila. Knockdown of LbEST-inte4 resulted in a substantial increase in the booklice susceptibility to malathion. Overexpression of LbEST-inte4 in Drosophila melanogaster significantly enhanced its malathion tolerance. Molecular modeling and docking analysis suggested potential interactions between LbEST-inte4 and malathion. When overexpressed LbEST-inte4 in Sf9 cells, a notable elevation in esterase activity and malathion tolerance was observed. HPLC analysis indicated that the LbEST-inte4 enzyme could effectively degrade malathion. Taken together, the upregulated LbEST-inte4 appears to contribute to malathion tolerance in L. bostrychophila by facilitating the depletion of malathion. This study elucidates the molecular mechanism underlying malathion detoxification and provides the foundations for the development of effective prevention and control measures against psocids.

中文翻译：

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珠被酯酶基因 LbEST-inte4 的过度表达可推断 Liposcelis bostrychophila（裸翅目：Liposcelididae）的马拉硫磷解毒作用

Liposcelis bostrychophila，俗称书虱，是世界范围内重要的储粮害虫。研究表明，书虱已经对几种杀虫剂产生了抗药性。在这项研究中，在L. bostrychophila中鉴定了表达上调的珠被酯酶基因LbEST-inte4。LbEST-inte4的敲除导致书虱对马拉硫磷的敏感性大幅增加。果蝇中LbEST-inte4的过表达显着增强了其马拉硫磷耐受性。分子建模和对接分析表明 LbEST-inte4 和马拉硫磷之间存在潜在的相互作用。当在 Sf9 细胞中过表达LbEST-inte4时，观察到酯酶活性和马拉硫磷耐受性显着升高。 HPLC分析表明LbEST-inte4酶可以有效降解马拉硫磷。总而言之，上调的LbEST-inte4似乎通过促进马拉硫磷的消耗而有助于L. bostrychophila的马拉硫磷耐受性。该研究阐明了马拉硫磷解毒的分子机制，为制定有效的针对psocids的预防和控制措施提供了基础。