RNAi plays a crucial role in insect gene function research and pest control field. Nonetheless, the variable efficiency of RNAi across diverse insects and off-target effects also limited its further application. In this study, we cloned six essential housekeeping genes from Solenopsis invicta and conducted RNAi experiments by orally administering dsRNA. Then, we found that mixing with liposomes significantly enhanced the RNAi efficiency by targeting for SiV-ATPaseE. Additionally, we observed a certain lethal effect of this dsRNA on queens by our established RNAi system. Furthermore, no strict sequence-related off-target effects were detected. Finally, the RNAi effect of large-scale bacteria expressing dsRNA was successfully confirmed for controlling S. invicta. In summary, this study established an RNAi system for S. invicta and provided a research template for the future development of nucleic acid drugs based on RNAi.

中文翻译：

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RNAi介导的红火蚁病虫害防治方法的建立

RNAi在昆虫基因功能研究和害虫防治领域发挥着至关重要的作用。尽管如此，RNAi在不同昆虫中的不同效率和脱靶效应也限制了其进一步应用。在这项研究中，我们从红火蚁中克隆了 6 个重要的管家基因，并通过口服 dsRNA 进行了 RNAi 实验。然后，我们发现与脂质体混合通过靶向SiV-ATPaseE显着提高了 RNAi 效率。此外，我们通过建立的 RNAi 系统观察到这种 dsRNA 对蜂王具有一定的致死作用。此外，没有检测到严格的序列相关的脱靶效应。最后，成功证实了表达dsRNA的大型细菌对控制S. invicta的RNAi效果。综上所述，本研究建立了S. invicta的RNAi系统，为未来开发基于RNAi的核酸药物提供了研究模板。