Accurate detection of multiple cardiovascular biomarkers is crucial for the timely screening of acute coronary syndrome (ACS) and differential diagnosis from acute aortic syndrome (AAS). Herein, an antibody microarray-based metal-enhanced fluorescence assay (AMMEFA) has been developed to quantitatively detect 7 cardiovascular biomarkers through the formation of a sandwich immunoassay on the poly(glycidyl methacrylate-co-2-hydroxyethyl methacrylate)-decorated GNR-modified slide (GNR@P(GMA-HEMA) slide). The AMMEFA exhibits high specificity and sensitivity, the linear ranges span 5 orders of magnitude, and the limits of detection (LODs) of cardiac troponin I (cTnI), heart-type fatty acid binding protein (H-FABP), C-reactive protein (CRP), copeptin, myoglobin, D-Dimer, and N-terminal pro-brain natriuretic peptide (NT-proBNP) reach 0.07, 0.2, 65.7, 0.6, 0.2, 8.3, and 0.3 pg mL^–1, respectively. To demonstrate its practicability, the AMMEFA has been applied to quantitatively analyze 7 cardiovascular biomarkers in 140 clinical plasma samples. In addition, the expression levels of cardiovascular biomarkers were analyzed by the least absolute shrinkage and selector operator (LASSO) regression, and the area under receiver operator characteristic curves (AUCs) of healthy donors (HDs), ACS patients, and AAS patients are 0.99, 0.98, and 0.97, respectively.

中文翻译：

---

通过基于抗体微阵列的金属增强荧光测定法定量检测多种心血管生物标志物


准确检测多种心血管生物标志物对于及时筛查急性冠脉综合征（ACS）以及与急性主动脉综合征（AAS）的鉴别诊断至关重要。在此，开发了一种基于抗体微阵列的金属增强荧光测定（AMMEFA），通过在聚（甲基丙烯酸缩水甘油酯-共-甲基丙烯酸2-羟乙酯）修饰的GNR修饰上形成夹心免疫测定来定量检测7种心血管生物标志物幻灯片（GNR@P（GMA-HEMA）幻灯片）。 AMMEFA 具有较高的特异性和灵敏度，线性范围跨越 5 个数量级，以及心肌肌钙蛋白 I (cTnI)、心脏型脂肪酸结合蛋白 (H-FABP)、C 反应蛋白的检测限 (LOD) (CRP)、和肽素、肌红蛋白、D-二聚体和 N 端脑钠肽前体 (NT-proBNP) 达到 0.07、0.2、65.7、0.6、0.2、8.3 和 0.3 pg mL ^–1 ， 分别。为了证明其实用性，AMMEFA 已用于定量分析 140 个临床血浆样本中的 7 种心血管生物标志物。此外，通过最小绝对收缩和选择算子（LASSO）回归分析心血管生物标志物的表达水平，健康供体（HD）、ACS患者和AAS患者的接受者算子特征曲线下面积（AUC）为0.99 、 0.98 和 0.97 分别。