Detection of procalcitonin (PCT) is crucial for the early identification of sepsis. PCT is primarily utilized in the multiple diagnosis of bacterial and viral illnesses along with to guide the application of antibiotics. Considering their advantages of high specificity and straightforward usage, electrochemical immunosensors offer significant application prospects in the detection of disease indicators. A dual-mode electrochemical immunosensor was constructed in this study to reliably identify PCT. In light of the synergistic effect of the dual-MOF derived heterostructure, the immunosensor demonstrating excellent square wave voltammetry (SWV) signals as well as significant catalytic activity for the HO redox process. In addition to maintaining a low detection limit (SWV: 0.31 fg/mL and i-t: 0.098 fg/mL), the immunosensor offers an extensive linear response range (0.000001–100 ng/mL). The excellent performance is on account of the introduction of the local on-site sulfurized dual-MOF heterostructure with abundant metal chalcogenides/MOF interfaces, which boosts the specific surface area, offers an abundance of active sites, enhances conductivity, and raises catalytic activity. Furthermore, the immunosensor exhibits outstanding specificity, stability and reproducibility for the determination of PCT in serum, which is of great crucial for the clinical screening and diagnosis of sepsis.

中文翻译：

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双模式无标记电化学免疫传感器，通过双 MOF 异质结构现场硫化超灵敏检测降钙素原


降钙素原（PCT）的检测对于脓毒症的早期识别至关重要。 PCT 主要用于细菌和病毒性疾病的多重诊断以及指导抗生素的应用。电化学免疫传感器具有特异性高、使用简单等优点，在疾病指标检测方面具有重要的应用前景。本研究构建了双模式电化学免疫传感器来可靠地识别 PCT。鉴于双 MOF 衍生异质结构的协同效应，免疫传感器表现出优异的方波伏安法 (SWV) 信号以及 H2O 氧化还原过程的显着催化活性。除了保持较低的检测限（SWV：0.31 fg/mL 和 i-t：0.098 fg/mL）外，免疫传感器还提供广泛的线性响应范围（0.000001–100 ng/mL）。优异的性能归功于引入具有丰富金属硫属化物/MOF界面的局部现场硫化双MOF异质结构，提高了比表面积，提供了丰富的活性位点，增强了电导率，并提高了催化活性。此外，该免疫传感器在测定血清中PCT方面表现出优异的特异性、稳定性和重现性，这对于脓毒症的临床筛查和诊断至关重要。