The detection of biomarkers is of great significance for medical diagnosis, food safety, environmental monitoring, and agriculture. However, bio-detection technology at present often necessitates complex instruments, expensive reagents, specialized expertise, and prolonged procedures, making it challenging to fulfill the demand for rapid, sensitive, user-friendly, and economical testing. In contrast, lateral flow strip (LFS) technology offers simple, fast, and visually accessible detection modality, allowing real-time analysis of clinical specimens, thus finding widespread utility across various domains. Within the realm of LFS, the application of aptamers as molecular recognition probes presents distinct advantages over antibodies, including cost-effectiveness, smaller size, ease of synthesis, and chemical stability. In recent years, aptamer-based LFS has found extensive application in qualitative, semi-quantitative, and quantitative detection across food safety, environmental surveillance, clinical diagnostics, and other domains. This review provided a concise overview of different aptamer screening methodologies, selection strategies, underlying principles, and procedural, elucidating their respective advantages, limitations, and applications. Additionally, we summarized recent strategies and mechanisms for aptamer-based LFS, such as the sandwich and competitive methods. Furthermore, we classified LFSs constructed based on aptamers, considering the rapid advancements in this area, and discussed their applications in biological and chemical detection. Finally, we delved into the current challenges and future directions in the development of aptamer and aptamer-based LFS. Although this review was not thoroughly, it would serve as a valuable reference for understanding the research progress of aptamer-based LFS and aid in the development of new types of aptasensors.

中文翻译：

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适配子筛选及侧流试纸条应用的全面综述：现状与未来展望


生物标志物的检测对于医学诊断、食品安全、环境监测、农业等具有重要意义。然而，目前的生物检测技术往往需要复杂的仪器、昂贵的试剂、专业知识和漫长的程序，这使得满足快速、灵敏、用户友好和经济的测试需求具有挑战性。相比之下，侧流试纸条 (LFS) 技术提供简单、快速且直观的检测方式，允许对临床标本进行实时分析，从而在各个领域得到广泛应用。在 LFS 领域，适体作为分子识别探针的应用与抗体相比具有明显的优势，包括成本效益、较小的尺寸、易于合成和化学稳定性。近年来，基于适配体的LFS在食品安全、环境监测、临床诊断等领域的定性、半定量和定量检测中得到了广泛的应用。本综述简要概述了不同的适体筛选方法、选择策略、基本原理和程序，阐明了它们各自的优点、局限性和应用。此外，我们总结了基于适配体的 LFS 的最新策略和机制，例如夹心法和竞争法。此外，考虑到该领域的快速进展，我们对基于适配体构建的LFS进行了分类，并讨论了它们在生物和化学检测中的应用。最后，我们深入探讨了适配体和基于适配体的LFS开发当前的挑战和未来方向。 虽然本文的综述并不全面，但对于了解基于适配体的LFS研究进展以及新型适配体传感器的开发具有一定的参考价值。