The development of simple methods for the isolation and quantification of exosomes in biological samples is important. By using the typical two-dimensional (2D) nanomaterials, graphene oxide (GO), the present work first studied the interaction of liposomes with the nanocomposites formed by adsorbing HRP on the GO surface and found the presence of liposomes led to the release of HRP from the GO surface to the solution phase triggering the luminol-HO chemiluminescence (CL) reaction to emit light. Benefiting from the similarity of exosomes to liposomes in both composition and morphology aspects, the GO-HRP nanocomposites with a mass ratio of 120:1 and 160:1 were employed for the quantitative detection of exosomes in 100-fold diluted serum samples. The whole detection process took about 15 min and as low as 3.2 × 10 particles μL of exosomes could be sensitively detected. In addition to GO-HRP nanocomposites, the CL responses of other nanocomposites obtained from adsorbing HRP on other 2D nanomaterials such as layered MoS for exosomes were also tested. MoS-HRP exhibited similar behavior and the LODs for the detection of exosomes were 5.8 × 10 particles μL. The proposed assays were a biomarker-independent quantitative method that achieved the quantification of exosomes in serum samples directly without an isolation process.

中文翻译：

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基于吸附在二维纳米材料上的辣根过氧化物酶的外泌体定量的简单化学发光方法


开发用于分离和定量生物样品中外泌体的简单方法非常重要。本工作利用典型的二维（2D）纳米材料氧化石墨烯（GO），首先研究了脂质体与GO表面吸附HRP形成的纳米复合材料的相互作用，发现脂质体的存在导致HRP的释放从GO表面到溶液相，触发鲁米诺-H2O化学发光（CL）反应发光。受益于外泌体与脂质体在组成和形态方面的相似性，采用质量比为120:1和160:1的GO-HRP纳米复合物用于100倍稀释血清样品中外泌体的定量检测。整个检测过程大约需要15分钟，可以灵敏地检测到低至3.2×10μL的外泌体颗粒。除了 GO-HRP 纳米复合材料之外，还测试了通过在其他 2D 纳米材料（例如用于外泌体的层状 MoS2）上吸附 HRP 获得的其他纳米复合材料的 CL 响应。 MoS-HRP 表现出类似的行为，检测外泌体的 LOD 为 5.8 × 10 个颗粒 μL。所提出的测定是一种独立于生物标志物的定量方法，无需分离过程即可直接实现血清样品中外泌体的定量。