DNA is commonly employed as a substrate for the building of artificial logic networks due to its excellent biocompatibility and programmability. Till now, DNA logic circuits are rapidly evolving to accomplish advanced operations. Nonetheless, nowadays, most DNA circuits remain to be disposable and lack of field programmability and thereby limits their practicability. Herein, inspired by the Configurable Logic Block (CLB), the CLB‐based erasable field‐programmable DNA circuit that uses clip strands as its operation‐controlling signals is presented. It enables users to realize diverse functions with limited hardware. CLB‐based basic logic gates (OR and AND) are first constructed and demonstrated their erasability and field programmability. Furthermore, by adding the appropriate operation‐controlling strands, multiple rounds of programming are achieved among five different logic operations on a two‐layer circuit. Subsequently, a circuit is successfully built to implement two fundamental binary calculators: half‐adder and half‐subtractor, proving that the design can imitate silicon‐based binary circuits. Finally, a comprehensive CLB‐based circuit is built that enables multiple rounds of switch among seven different logic operations including half‐adding and half‐subtracting. Overall, the CLB‐based erasable field‐programmable circuit immensely enhances their practicability. It is believed that design can be widely used in DNA logic networks due to its efficiency and convenience.

中文翻译：

---

基于可配置逻辑块的可擦除和现场可编程 DNA 电路

由于其优异的生物相容性和可编程性，DNA通常被用作构建人工逻辑网络的基质。迄今为止，DNA 逻辑电路正在迅速发展以完成高级操作。尽管如此，如今大多数 DNA 电路仍然是一次性的并且缺乏现场可编程性，从而限制了它们的实用性。在此，受可配置逻辑块（CLB）的启发，提出了基于CLB的可擦除现场可编程DNA电路，该电路使用剪辑链作为其操作控制信号。它使用户能够用有限的硬件实现多样化的功能。首先构建了基于 CLB 的基本逻辑门（OR 和 AND）并展示了它们的可擦除性和现场可编程性。此外，通过添加适当的操作控制链，可以在双层电路上的五种不同逻辑操作之间实现多轮编程。随后，成功构建了一个电路来实现两个基本的二进制计算器：半加器和半减器，证明该设计可以模仿硅基二进制电路。最后，构建了一个基于 CLB 的综合电路，该电路能够在七种不同的逻辑运算（包括半加和半减）之间进行多轮切换。总体而言，基于 CLB 的可擦除现场可编程电路极大地增强了其实用性。人们相信，设计因其高效和便利而可以广泛应用于DNA逻辑网络。