Real‐time monitoring the therapeutic process of sonodynamic therapy (SDT) is essential to optimize the treatment course in time and eventually improve the efficacy. The generation of singlet oxygen (1O2) is a quintessential characteristic of SDT, which permits non‐invasive monitoring of SDT by real‐time imaging of 1O2 inside the tumor. Nonetheless, the majority of probes are unable to measure 1O2 in real time because of its short half‐life and strong oxidative capacity. Here, the study constructs a ratiometric nanoplatform (DTPI) utilizing two fluorescent probes and the sonosensitizer TiO2. The poisonous 1O2 generated by DTPI following ultrasonic (US) radiation efficiently destroys tumor cells. The structural disruption of fluorescent dye IR‐1061 by 1O2 leads to a reduction in the DTPI fluorescence signal at 1100 nm, while US radiation has no impact on the fluorescence signal at 1550 nm. Thus, DTPI provides a precise and consistent reflection of the treatment efficacy at the tumor site, leveraging the ratiometric fluorescence signal and variations in oxygen content throughout the treatment process. This ratiometric‐fluorescence‐based reflection strategy establishes an effective and dependable platform for the real‐time monitoring and assessment of the cancer therapeutic effect through ratiometric probes.

中文翻译：

---

用于实时监测和评估癌症声动力治疗效果的 NIR-II 比率荧光纳米平台

实时监测声动力疗法（SDT）的治疗过程对于及时优化治疗过程并最终提高疗效至关重要。单线态氧的产生（1氧2）是 SDT 的一个典型特征，它允许通过实时成像对 SDT 进行非侵入性监测1氧2肿瘤内部。尽管如此，大多数探针无法测量1氧2由于其半衰期短和氧化能力强，因此可以实时进行。在这里，该研究利用两种荧光探针和声敏剂 TiO 构建了比率纳米平台 (DTPI)2。有毒的1氧2超声波（US）辐射后由 DTPI 产生，可有效破坏肿瘤细胞。荧光染料 IR-1061 的结构破坏1氧2导致 1100 nm 处的 DTPI 荧光信号减少，而 US 辐射对 1550 nm 处的荧光信号没有影响。因此，DTPI 利用比例荧光信号和整个治疗过程中氧含量的变化，提供了肿瘤部位治疗效果的精确且一致的反映。这种基于比率荧光的反射策略为通过比率探针实时监测和评估癌症治疗效果建立了一个有效且可靠的平台。