Nasopharyngeal carcinoma (NPC) is a prevalent malignancy that usually occurs among the nose and throat. Due to mild initial symptoms, most patients are diagnosed in the late stage, and the recurrence rate of tumors is high, resulting in many deaths every year. Traditional radiotherapy and chemotherapy are prone to causing drug resistance and significant side effects. Therefore, searching for new bioactive drugs including anticancer peptides is necessary and urgent. LVTX-8 is a peptide toxin synthesized from the cDNA library of the spider Lycosa vittata, which is consisting of 25 amino acids. In this study, a series of in vitro cell experiments such as cell toxicity, colony formation, and cell migration assays were performed to exam the anticancer activity of LVTX-8 in NPC cells (5-8F and CNE-2). The results suggested that LVTX-8 significantly inhibited cell proliferation and migration of NPC cells. To find the potential molecular targets for the anticancer capability of LVTX-8, high-throughput proteomic and bioinformatics analysis were conducted on NPC cells. The results identified EXOSC1 as a potential target protein with significantly differential expression levels under LVTX-8+/LVTX-8– conditions. The results in this research indicate that spider peptide toxin LVTX-8 exhibits significant anticancer activity in NPC, and EXOSC1 may serve as a target protein for its anticancer activity. These findings provide a reference for the development of new therapeutic drugs for NPC and offer new ideas for the discovery of biomarkers related to NPC diagnosis. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (https://proteomecentral.proteomexchange.org) via the iProX partner repository with the data set identifier PXD050542.

中文翻译：

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比较蛋白质组学鉴定 EXOSC1 是鼻咽癌细胞中抗癌肽 LVTX-8 的靶蛋白

鼻咽癌（NPC）是一种常见的恶性肿瘤，通常发生在鼻子和喉咙中。由于初期症状较轻，大多数患者确诊时已是晚期，肿瘤复发率较高，每年导致多人死亡。传统的放疗和化疗容易产生耐药性，且副作用明显。因此，寻找包括抗癌肽在内的新型生物活性药物是必要且紧迫的。 LVTX-8是从蜘蛛Lycosa vittata的cDNA文库中合成的肽毒素，由25个氨基酸组成。在本研究中，进行了一系列体外细胞实验，例如细胞毒性、集落形成和细胞迁移测定，以检测LVTX-8在NPC细胞（5-8F和CNE-2）中的抗癌活性。结果表明，LVTX-8显着抑制鼻咽癌细胞的增殖和迁移。为了寻找 LVTX-8 抗癌能力的潜在分子靶点，对鼻咽癌细胞进行了高通量蛋白质组学和生物信息学分析。结果确定 EXOSC1 是一种潜在的靶蛋白，在 LVTX-8+/LVTX-8- 条件下表达水平存在显着差异。本研究结果表明蜘蛛肽毒素LVTX-8在鼻咽癌中表现出显着的抗癌活性，而EXOSC1可能作为其抗癌活性的靶蛋白。这些研究结果为鼻咽癌新治疗药物的开发提供了参考，并为鼻咽癌诊断相关生物标志物的发现提供了新思路。质谱蛋白质组学数据已通过 iProX 合作伙伴存储库存入 ProteomeXchange 联盟 (https://proteomecentral.proteomexchange.org)，数据集标识符为 PXD050542。