Hydrodynamic dimension (HD) is the primary indicator of the size of bioconjugated particles and biomolecules. It is an important parameter in the study of solid–liquid two-phase dynamics. HD dynamic monitoring is crucial for precise and customized medical research as it enables the investigation of the continuous changes in the physicochemical characteristics of biomolecules in response to external stimuli. However, current HD measurements based on Brownian motion, such as dynamic light scattering (DLS), are inadequate for meeting the polydisperse sample demands of dynamic monitoring. In this paper, we propose MMQCM method samples of various types and HD dynamic monitoring. An alternating magnetic field of frequency ω_m excites biomolecule-magnetic bead particles (bioMBs) to generate magnetization motion, and the quartz crystal microbalance (QCM) senses this motion to provide HD dynamic monitoring. Specifically, the magnetization motion is modulated onto the thickness-shear oscillation of the QCM at the frequency ω_q. By analysis of the frequency spectrum of the QCM output signal, the ratio of the magnitudes of the real and imaginary parts of the components at frequency ω_q ± 2ω_m is extracted to characterize the particle size. Using the MMQCM approach, we successfully evaluated the size of bioMBs with different biomolecule concentrations. The 30 min HD dynamic monitoring was implemented. An increase of ∼10 nm in size was observed upon biomolecular structural stretching. Subsequently, the size of bioMBs gradually reduced due to the continuous dissociation of biomolecules, with a total reduction of 20∼40 nm. This HD dynamic monitoring demonstrates that the release of biomolecules can be regulated by controlling the duration of magnetic stimulation, providing valuable insights and guidance for controlled drug release in personalized precision medicine.

中文翻译：

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通过石英晶体微天平上的磁化运动动态监测生物分子流体动力学尺寸


流体动力学尺寸（HD）是生物共轭颗粒和生物分子尺寸的主要指标。它是固液两相动力学研究中的重要参数。高清动态监测对于精确和定制的医学研究至关重要，因为它可以研究生物分子理化特性响应外部刺激的连续变化。然而，当前基于布朗运动的高清测量，例如动态光散射（DLS），不足以满足动态监测的多分散样品需求。在本文中，我们提出了各种类型的MMQCM方法示例和高清动态监控。频率为ω _m 的交变磁场激发生物分子磁珠颗粒（bioMB）产生磁化运动，石英晶体微天平（QCM）感测这种运动以提供高清动态监测。具体来说，磁化运动被调制到频率为 ω _q 的 QCM 的厚度剪切振荡上。通过分析 QCM 输出信号的频谱，提取频率 ω _q ± 2ω _m 分量的实部和虚部幅值比来表征颗粒大小。使用 MMQCM 方法，我们成功评估了不同生物分子浓度的 bioMB 的大小。实施30分钟高清动态监控。生物分子结构拉伸后观察到尺寸增加了~10 nm。随后，由于生物分子的不断解离，bioMBs的尺寸逐渐减小，总共减小了20∼40 nm。 这种高清动态监测表明，可以通过控制磁刺激的持续时间来调节生物分子的释放，为个性化精准医疗中的受控药物释放提供有价值的见解和指导。