Time-critical transcriptional events in the immune microenvironment are important for response to immune checkpoint blockade (ICB), yet these events are difficult to characterise and remain incompletely understood. Here, we present whole tumor RNA sequencing data in the context of treatment with ICB in murine models of AB1 mesothelioma and Renca renal cell cancer. We sequenced 144 bulk RNAseq samples from these two cancer types across 4 time points prior and after treatment with ICB. We also performed single-cell sequencing on 12 samples of AB1 and Renca tumors an hour before ICB administration. Our samples were equally distributed between responders and non-responders to treatment. Additionally, we sequenced AB1-HA mesothelioma tumors treated with two sample dissociation protocols to assess the impact of these protocols on the quality transcriptional information in our samples. These datasets provide time-course information to transcriptionally characterize the ICB response and provide detailed information at the single-cell level of the early tumor microenvironment prior to ICB therapy.

免疫微环境中的时间关键转录事件对于免疫检查点封锁（ICB）的反应非常重要，但这些事件很难表征并且仍未完全了解。在这里，我们展示了在 AB1 间皮瘤和 Renca 肾细胞癌小鼠模型中使用 ICB 治疗的整个肿瘤 RNA 测序数据。我们对 ICB 治疗前后 4 个时间点的 144 个来自这两种癌症类型的批量 RNAseq 样本进行了测序。我们还在 ICB 给药前一小时对 12 个 AB1 和 Renca 肿瘤样本进行了单细胞测序。我们的样本在治疗有反应者和无反应者之间平均分配。此外，我们对用两种样品解离方案治疗的 AB1-HA 间皮瘤肿瘤进行了测序，以评估这些方案对我们样品中转录信息质量的影响。这些数据集提供时间进程信息来转录表征 ICB 反应，并提供 ICB 治疗前早期肿瘤微环境的单细胞水平的详细信息。