Inspired by the attractive advantages of near-infrared (NIR) fluorescent detection, a novel NIR fluorescent probe MCP-Cor-DNBS, based on corrole skeleton bearing 2,4-dinitrobenzenesulfonyl ester group, was rationally designed and developed for effective detection of Cys. The probe toward Cys displayed bright red emission (684 nm), distinct color response (from green to slight yellow), short response time (80 s), large Stokes shift (234 nm), high selectivity and sensitivity (LOD = 37 nM). The sensing mechanism was illustrated by fluorescence spectroscopy, MS analysis and DFT calculations. Furthermore, MCP-Cor-DNBS could not only be used for quantitative determination of Cys in food samples, but also be successfully used for imaging Cys in HeLa cells and zebrafish.

中文翻译：

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基于咔咯荧光基团的近红外开启荧光探针特异性检测半胱氨酸及其应用


受近红外（NIR）荧光检测的诱人优势的启发，合理设计并开发了一种基于带有2,4-二硝基苯磺酰酯基团的咔咯骨架的新型NIR荧光探针MCP-Cor-DNBS，用于有效检测Cys。 Cys 探针显示出明亮的红色发射 (684 nm)、独特的颜色响应（从绿色到淡黄色）、响应时间短 (80 s)、斯托克斯位移大 (234 nm)、高选择性和灵敏度 (LOD = 37 nM) 。通过荧光光谱、MS 分析和 DFT 计算说明了传感机制。此外，MCP-Cor-DNBS不仅可以用于食品样品中Cys的定量测定，还可以成功用于HeLa细胞和斑马鱼中Cys的成像。