The development of supramolecular cucurbit[7]uril-based chemosensors for the detection of bioanalytes in biofluids such as untreated human serum and inside cells is a challenging task due to competition with proteins and inorganic salts. In this contribution, we show that the encapsulation of cucurbit[7]uril-based chemosensors in polymersomes can prevent deactivation, rendering the chemosensors operational in human serum and inside cells. We found that polymersomes with a hydrophilic poly-[N,N-dimethylacrylamide] corona, especially those smaller than 200 nm, exhibit greater permeability to small bioactive molecules compared with polymersomes with a bulkier poly(ethylene glycol) corona. Furthermore, analytes characterized by intermediate lipophilicity, low charge density, and a rigid structure display enhanced permeability through the polymersomes. The polymer membrane serves as a selective filter that allows small molecules to pass through a chemosensor while larger proteins are held outside the polymersome. In addition to providing a new approach for stabilizing chemosensors in protein-rich media, this study underscores the potential utility of polymersome-encapsulated chemosensors in investigating membrane permeability.

中文翻译：

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聚合物囊泡封装的化学传感器：生物流体适用的葫芦[7]尿素指示剂位移测定的新设计策略

由于与蛋白质和无机盐的竞争，开发基于葫芦[7]脲的超分子化学传感器用于检测生物流体（例如未经处理的人血清和细胞内）中的生物分析物是一项具有挑战性的任务。在这篇文章中，我们表明，将基于葫芦[7]脲的化学传感器封装在聚合物囊泡中可以防止失活，使化学传感器在人血清和细胞内发挥作用。我们发现，与具有较大聚乙二醇冠的聚合物囊泡相比，具有亲水性聚[ N,N-二甲基丙烯酰胺]冠的聚合物囊泡，尤其是那些小于200 nm的聚合物囊泡，对小生物活性分子表现出更大的渗透性。此外，具有中等亲脂性、低电荷密度和刚性结构特征的分析物显示出通过聚合物囊泡的增强的渗透性。聚合物膜充当选择性过滤器，允许小分子通过化学传感器，而较大的蛋白质则被保留在聚合物囊泡之外。除了提供一种在富含蛋白质的介质中稳定化学传感器的新方法之外，这项研究还强调了聚合物囊泡封装的化学传感器在研究膜渗透性方面的潜在用途。