Addressing bone defects represents a significant challenge to public health. Localized delivery of growth factor has emerged as promising approach for bone regeneration. However, the clinical application of Platelet-Derived Growth Factor (PDGF) is hindered by its high cost and short half-life. In this work, we introduce the application of PDGF-mimicking peptide () hydrogels for calvarial defect restoration, showcasing their remarkable effectiveness. Through osteogenic differentiation assays and q-PCR analyses, we demonstrate 's substantial capacity to enhance osteogenic differentiation of bone marrow mesenchymal stem cell (BMSC), leading to increased expression of crucial osteogenic genes. Further molecular mechanistic investigations reveal 's activation of the PI3K-AKT-mTOR signaling pathway, a key element of its osteogenic effect. experiments utilizing a rat calvaria critical-sized defect model underscore the hydrogels' exceptional ability to accelerate new bone formation, thereby significantly advancing the restoration of calvaria defects. This research provides a promising bioactive material for bone tissue regeneration.

中文翻译：

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使用 PDGF-BB 模拟肽水凝胶增强颅骨缺损修复

解决骨缺陷是对公共卫生的重大挑战。生长因子的局部递送已成为骨再生的有前途的方法。然而，血小板衍生生长因子（PDGF）的临床应用因其成本高、半衰期短而受到阻碍。在这项工作中，我们介绍了PDGF模拟肽（）水凝胶在颅骨缺损修复中的应用，展示了其显着的功效。通过成骨分化测定和 q-PCR 分析，我们证明了 其增强骨髓间充质干细胞 (BMSC) 成骨分化的巨大能力，从而导致关键成骨基因表达增加。进一步的分子机制研究揭示了 PI3K-AKT-mTOR 信号通路的激活，这是其成骨作用的关键要素。利用大鼠颅盖临界尺寸缺损模型进行的实验强调了水凝胶加速新骨形成的卓越能力，从而显着促进颅盖缺损的修复。这项研究为骨组织再生提供了一种有前景的生物活性材料。