This study aimed to convert wastes generated during the extraction of oil from seeds, including the fruit shell (COFS) and seed cake (COSC), into fermentable feedstocks using pretreatment and enzymatic hydrolysis processes. The application of a green acid catalyst in the thermochemical pretreatment of the COFS and COSC mixture yielded the highest sugar of 28 g/L. Solid-state fermentation (SSF) was conducted on-site using various waste biomass substrates to produce enzymes for the saccharification process. This study found that COFS was the ideal substrate for generating SSF enzymes capable of breaking down lignocellulosic biomass. A novel enzymatic hydrolysis strategy was developed to minimize water consumption and enhance substrate utilization efficiency. By fine-tuning the enzymatic hydrolysis process, an additional sugar of 20.58 g/L was achieved using thermochemically pretreated solid residues and SSF enzymes. Standard techniques were used to analyze the raw materials, residual solids, and hydrolysates to assess the efficiency of each processing step. In general, the use of SSF enzymes and green pretreatment methods, reduction of water consumption in the process, and implementation of an efficient biomass utilization approach, enhance the overall efficiency and eco-friendliness of this study.

中文翻译：

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最大限度地利用油茶籽油提取残渣中的可发酵原料：绿色预处理和酶水解，实现有效增值

本研究旨在利用预处理和酶水解工艺，将种子（包括果壳 (COFS) 和籽饼 (COSC)）提取油过程中产生的废物转化为可发酵原料。应用绿色酸催化剂对 COFS 和 COSC 混合物进行热化学预处理，得到最高糖度 28 g/L。使用各种废弃生物质底物在现场进行固态发酵（SSF），以生产用于糖化过程的酶。这项研究发现，COFS 是产生能够分解木质纤维素生物质的 SSF 酶的理想底物。开发了一种新颖的酶水解策略，以最大限度地减少水消耗并提高底物利用效率。通过微调酶水解过程，使用热化学预处理的固体残渣和 SSF 酶获得了 20.58 g/L 的额外糖分。使用标准技术分析原材料、残留固体和水解产物，以评估每个加工步骤的效率。总的来说，SSF酶和绿色预处理方法的使用、过程中水消耗的减少以及高效生物质利用方法的实施，提高了本研究的整体效率和生态友好性。