Regular exercise promotes whole-body health and prevents disease, but the underlying molecular mechanisms are incompletely understood^1,2,3. Here, the Molecular Transducers of Physical Activity Consortium⁴ profiled the temporal transcriptome, proteome, metabolome, lipidome, phosphoproteome, acetylproteome, ubiquitylproteome, epigenome and immunome in whole blood, plasma and 18 solid tissues in male and female Rattus norvegicus over eight weeks of endurance exercise training. The resulting data compendium encompasses 9,466 assays across 19 tissues, 25 molecular platforms and 4 training time points. Thousands of shared and tissue-specific molecular alterations were identified, with sex differences found in multiple tissues. Temporal multi-omic and multi-tissue analyses revealed expansive biological insights into the adaptive responses to endurance training, including widespread regulation of immune, metabolic, stress response and mitochondrial pathways. Many changes were relevant to human health, including non-alcoholic fatty liver disease, inflammatory bowel disease, cardiovascular health and tissue injury and recovery. The data and analyses presented in this study will serve as valuable resources for understanding and exploring the multi-tissue molecular effects of endurance training and are provided in a public repository (https://motrpac-data.org/).

定期锻炼可以促进全身健康并预防疾病，但其潜在的分子机制尚不完全清楚^1,2,3。在此，体能活动联盟分子传感器^{4分析了雄性和雌性}褐家鼠在八周的耐力过程中全血、血浆和 18 个实体组织中的时间转录组、蛋白质组、代谢组、脂质组、磷酸蛋白质组、乙酰蛋白质组、泛素蛋白质组、表观基因组和免疫组。运动训练。由此产生的数据概要包含 19 个组织、25 个分子平台和 4 个训练时间点的 9,466 项检测。鉴定出数以千计的共享和组织特异性分子改变，并在多个组织中发现了性别差异。时间多组学和多组织分析揭示了对耐力训练适应性反应的广泛生物学见解，包括免疫、代谢、应激反应和线粒体途径的广泛调节。许多变化与人类健康相关，包括非酒精性脂肪肝、炎症性肠病、心血管健康以及组织损伤和恢复。本研究中提供的数据和分析将作为理解和探索耐力训练的多组织分子效应的宝贵资源，并在公共存储库中提供（https://motrpac-data.org/）。