The objective of the study was to independently validate a calibrated commercial handheld near infrared (NIR) spectroscopic device and test its repeatability over time using phenotypically diverse populations of Australian lamb. Validation testing in eight separate data sub-groups ( = 1591 carcasses overall) demonstrated that the NIR device had moderate precision (R = 0.4–0.64, RMSEP = 0.70–1.22%) but fluctuated in accuracy between experimental site demonstrated by variable slopes (0.50–0.94) and biases (−0.86–0.02). The repeatability experiment ( = 10 carcasses) showed that time to scan post quartering affected NIR measurement from 0 to 24 h ( < 0.001). On average, NIR IMF% was 0.97% lower (P < 0.001) at 24 h (4.01% ± 0.166), compared to 0 h. There was no difference ( > 0.05) between Time 0 and 1 h or Time 0 and 4 h or between replicate scans within each time point. This study demonstrated the SOMA NIR device could predict lamb chemical IMF% with moderate precision and accuracy, however additional work is required to understand how loin preparation, blooming and surface hydration affect NIR measurement.

中文翻译：

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手持式近红外分光光度计测量澳大利亚羔羊化学肌内脂肪的验证


该研究的目的是独立验证经过校准的商用手持式近红外 (NIR) 光谱设备，并使用表型多样化的澳大利亚羔羊群体测试其随时间的重复性。在八个独立的数据子组（总共 = 1591 具屠体）中进行的验证测试表明，NIR 设备具有中等精度（R = 0.4–0.64，RMSEP = 0.70–1.22%），但不同斜率（0.50）表明实验地点之间的准确度存在波动。 –0.94）和偏差（−0.86–0.02）。重复性实验（= 10 具屠体）表明，分割后的扫描时间会影响 0 至 24 小时的 NIR 测量 (< 0.001)。平均而言，与 0 小时相比，24 小时时 NIR IMF% 降低了 0.97% (P < 0.001) (4.01% ± 0.166)。时间 0 和 1 小时或时间 0 和 4 小时之间或每个时间点内的重复扫描之间没有差异 (> 0.05)。这项研究表明，SOMA NIR 设备可以以中等精度和准确度预测羊肉化学成分 IMF%，但需要做更多工作来了解腰肉准备、开花和表面水合如何影响 NIR 测量。