A microbial fuel cell with a mature pure-culture electroactive biofilm was used for performance optimization by making rapid changes to experimental parameters in microchannels while monitoring their effect using linear sweep voltammetry. A systematic investigation of polarization behavior and evaluation of system resistivity provided important figures of merit and mechanistic insights on the effects of flow rates, concentrations, and temperature. After individual parameters were optimized, a synergistic effect was observed by applying optimal parameters together, resulting in improved current and maximum power densities, compared to stable values at unoptimized conditions. Continued acclimation for just 2 days under these conditions resulted in further improvements to anode area-normalized current and power maxima (10.49 ± 0.23 A m and 2.48 ± 0.27 W m), which are among the highest reported in the literature for a microfluidic MFC. In keeping with other accepted normalization protocol using the area separating anode and cathode chambers, the output densities were 64 A m and 15 W m, which are near the theoretical maximum outputs achievable with an MFC system.

中文翻译：

---

使用线性扫描伏安法和微流体对微生物燃料电池输出进行多参数优化

使用具有成熟的纯培养电活性生物膜的微生物燃料电池，通过快速改变微通道中的实验参数，同时使用线性扫描伏安法监测其效果来优化性能。对极化行为的系统研究和系统电阻率的评估提供了关于流速、浓度和温度影响的重要品质因数和机制见解。优化各个参数后，通过一起应用最佳参数观察到协同效应，与未优化条件下的稳定值相比，电流和最大功率密度得到改善。在这些条件下持续适应仅 2 天，就可以进一步改善阳极面积归一化电流和功率最大值（10.49 ± 0.23 A m 和 2.48 ± 0.27 W m），这是微流体 MFC 文献中报道的最高值之​​一。与使用分隔阳极室和阴极室的区域的其他公认的归一化协议保持一致，输出密度为 64 A m 和 15 W m ，这接近 MFC 系统可实现的理论最大输出。