Removal of Exogenous Stimuli Reveals a Canalization of Circadian Physiology in a Vertically Migrating Copepod,Journal of Proteome Research

当前位置： X-MOL 学术 › J. Proteome Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Removal of Exogenous Stimuli Reveals a Canalization of Circadian Physiology in a Vertically Migrating Copepod
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2024-05-01 , DOI: 10.1021/acs.jproteome.4c00086
Emma Timmins-Schiffman ₁ , Amy E Maas ₂ , Rayhan Khanna _{1,

3} , Leocadio Blanco-Bercial ₂ , Eric Huang _{1,

4} , Brook L Nunn ₁

Affiliation

Diel rhythms are observed across taxa and are important for maintaining synchrony between the environment and organismal physiology. A striking example of this is the diel vertical migration undertaken by zooplankton, some of which, such as the 5 mm-long copepod Pleuromamma xiphias (P. xiphias), migrate hundreds of meters daily between the surface ocean and deeper waters. Some of the molecular pathways that underlie the expressed phenotype at different stages of this migration are entrained by environmental variables (e.g., day length and food availability), while others are regulated by internal clocks. We identified a series of proteomic biomarkers that vary across ocean DVM and applied them to copepods incubated in 24 h of darkness to assess circadian control. The dark-incubated copepods shared some proteomic similarities to the ocean-caught copepods (i.e., increased abundance of carbohydrate metabolism proteins at night). Shipboard-incubated copepods demonstrated a clearer distinction between night and day proteomic profiles, and more proteins were differentially abundant than in the in situ copepods, even in the absence of the photoperiod and other environmental cues. This pattern suggests that there is a canalization of rhythmic diel physiology in P. xiphias that reflects likely circadian clock control over diverse molecular pathways.

中文翻译：

去除外源刺激揭示了垂直迁移桡足类昼夜节律生理学的管道化

在整个分类单元中都观察到昼夜节律，对于维持环境和生物生理学之间的同步非常重要。一个引人注目的例子是浮游动物进行的昼夜垂直迁移，其中一些浮游动物，例如 5 毫米长的桡足类Pleuromamma xiphias ( P. xiphias )，每天在表层海洋和更深的水域之间迁移数百米。在这种迁移的不同阶段表达表型的一些分子途径受到环境变量（例如日长和食物可用性）的影响，而其他分子途径则受到内部时钟的调节。我们鉴定了一系列在海洋 DVM 中各不相同的蛋白质组生物标志物，并将它们应用于在 24 小时黑暗中孵化的桡足类动物，以评估昼夜节律控制。黑暗孵化的桡足类与海洋捕获的桡足类有一些蛋白质组相似之处（即夜间碳水化合物代谢蛋白的丰度增加）。船上孵化的桡足类动物在夜间和白天的蛋白质组谱之间表现出更清晰的区别，并且即使在没有光周期和其他环境线索的情况下，与原位桡足类动物相比，更多的蛋白质丰度存在差异。这种模式表明，剑齿鱼中节律性昼夜生理学存在管道化，这反映了可能对不同分子途径的生物钟控制。

更新日期：2024-05-01

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11