Peanut allergen monitoring is currently an effective strategy to avoid allergic diseases, while food matrix interference is a critical challenge during detection. Here, we developed an antifouling surface plasmon resonance sensor (SPR) with stratified zwitterionic peptides, which provides both excellent antifouling and sensing properties. The antifouling performance was measured by the SPR, which showed that stratified peptide coatings showed much better protein resistance, reaching ultralow adsorption levels (<5 ng/cm²). Atomic force microscopy was used to further analyze the antifouling mechanism from a mechanical perspective, which demonstrated lower adsorption forces on hybrid peptide coatings, confirming the better antifouling performance of stratified surfaces. Moreover, the recognition of peanut allergens in biscuits was performed using an SPR with high efficiency and appropriate recovery results (98.2–112%), which verified the feasibility of this assay. Therefore, the fabrication of antifouling sensors with stratified zwitterionic peptides provides an efficient strategy for food safety inspection.

中文翻译：

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制备具有分层两性离子肽的防污表面等离子体共振传感器，用于高效检测饼干中的花生过敏原

花生过敏原监测是目前避免过敏性疾病的有效策略，而食品基质干扰是检测过程中的关键挑战。在这里，我们开发了一种具有分层两性离子肽的防污表面等离子共振传感器（SPR），它具有优异的防污和传感性能。通过SPR测量防污性能，结果表明分层肽涂层表现出更好的蛋白质耐受性，达到超低吸附水平（<5 ng/cm ²）。利用原子力显微镜从力学角度进一步分析其防污机理，结果表明杂化肽涂层具有较低的吸附力，证实了分层表面具有更好的防污性能。此外，使用SPR对饼干中的花生过敏原进行了识别，效率高，回收率合适（98.2-112%），验证了该测定的可行性。因此，利用分层两性离子肽制备防污传感器为食品安全检测提供了一种有效的策略。