Enzymatic processes coupled with continuous microfluidic techniques have significant application prospects in pharmaceuticals and fine chemicals. It is important to stably load enzymes in microreactors. This study reported a sol–gel method for preparing wall-coated immobilized enzyme microreactors (W-IMERs) by entrapment of Candida antarctica lipase B (CALB). The W-IMERs were simply constructed in one step by a flow coating process in a capillary. Compared with the free enzyme, the tolerance of immobilized enzyme to ethanol and temperature is significantly improved, and the yield of immobilized enzyme activity is 76.12% in the batch reactor. In W-IMERs, the yield of immobilized enzyme activity increased to 82.95%. The W-IMERs maintained stable activity in 10 cycles for up to 30 days. A CFD model was also established to explore the relationship between the internal reaction and the mass transfer in W-IMERs. The relationship between the internal and external diffusion limits of W-IMERs and the overall reaction rate was analyzed by dimensionless numbers. The results indicated that sol–gel flow coating is a simple and effective method for the preparation of enzymatic microreactors. An in-depth understanding of the relationship between diffusion limitation and reaction rate will have a significant impact on the overall performance of enzyme-catalyzed microreactors.

中文翻译：

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表面固定化酶在毛细管微反应器中的酶促反应

酶促过程与连续微流控技术相结合在制药和精细化学品领域具有重要的应用前景。在微反应器中稳定地加载酶非常重要。本研究报告了一种通过包埋南极假丝酵母脂肪酶 B (CALB ) 制备壁包被固定化酶微反应器 (W-IMER) 的溶胶-凝胶方法。 W-IMER 通过毛细管中的流涂工艺一步简单地构建。与游离酶相比，固定化酶对乙醇和温度的耐受性显着提高，间歇式反应器中固定化酶活力产率为76.12%。在W-IMERs中，固定化酶活性的产率提高到82.95%。 W-IMER 在 10 个周期中保持稳定的活性长达 30 天。还建立了 CFD 模型来探讨 W-IMER 中内部反应与传质之间的关系。通过无量纲数分析了W-IMER的内部和外部扩散极限与总反应速率之间的关系。结果表明，溶胶-凝胶流涂是一种简单有效的酶微反应器制备方法。深入了解扩散限制与反应速率之间的关系将对酶催化微反应器的整体性能产生重大影响。