This study aimed to discover the potential of Medicago sativa-derived fungal endophytes as a prospective source of bioactive metabolites. In the present study, three different strains of fungal endophyte Aspergillus terreus were isolated from leaves L, roots T and stems St of Medicago sativa to explore their biological and chemical diversity. These isolated fungi were exposed to different fermentation conditions by adding various chemical elicitors to their solid fermentation media. According to LC-HRESIMS-based metabolomics and multivariate analysis, each chemical treatment had a different effect on the chemical profiles of the fungi. Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) proposed several compounds with anticancer action against MCF-7 (a human breast cancer cell line) and MDA-MB-231 (a human epithelial breast cancer cell line).

中文翻译：

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苜蓿源性内生真菌的代谢组学分析及其生物活性评价

本研究旨在发现紫花苜蓿衍生的内生真菌作为生物活性代谢物的潜在来源的潜力。本研究从苜蓿叶 L、根 T 和茎 St 中分离出三种不同菌株的内生真菌土曲霉(Aspergillus terreus)，以探索其生物和化学多样性。通过向其固体发酵培养基中添加各种化学激发子，将这些分离的真菌暴露于不同的发酵条件。根据基于 LC-HRESIMS 的代谢组学和多变量分析，每种化学处理对真菌的化学特征都有不同的影响。潜在结构正交投影判别分析 (OPLS-DA) 提出了几种对 MCF-7（人类乳腺癌细胞系）和 MDA-MB-231（人类上皮乳腺癌细胞系）具有抗癌作用的化合物。