Neurodevelopmental cell communication plays a crucial role in neuroblastoma prognosis. However, determining the impact of these communication pathways on prognosis is challenging due to limited sample sizes and patchy clinical survival information of single cell RNA-seq data. To address this, we have developed the cell communication pathway prognostic model (CCPPM) in this study. CCPPM involves the identification of communication pathways through single-cell RNA-seq data, screening of prognosis-significant pathways using bulk RNA-seq data, conducting functional and attribute analysis of these pathways, and analyzing the post-effects of communication within these pathways. By employing the CCPPM, we have identified ten communication pathways significantly influencing neuroblastoma, all related to axongenesis and neural projection development, especially the BMP7-(BMPR1B-ACVR2B) communication pathway was found to promote tumor cell migration by activating the transcription factor SMAD1 and regulating UNK and MYCBP2. Notably, BMP7 expression was higher in neuroblastoma samples with distant metastases. In summary, CCPPM offers a novel approach to studying the influence of cell communication pathways on disease prognosis and identified detrimental communication pathways related to neurodevelopment.

中文翻译：

---

细胞通讯途径预后模型确定了神经母细胞瘤中有害的神经发育途径

神经发育细胞通讯在神经母细胞瘤预后中起着至关重要的作用。然而，由于单细胞 RNA-seq 数据的样本量有限和临床生存信息不完整，确定这些通讯途径对预后的影响具有挑战性。为了解决这个问题，我们在本研究中开发了细胞通讯途径预后模型（CCPPM）。 CCPPM 涉及通过单细胞 RNA-seq 数据识别通讯途径，使用批量 RNA-seq 数据筛选对预后有意义的途径，对这些途径进行功能和属性分析，并分析这些途径内通讯的后效应。通过CCPPM，我们发现了10条对神经母细胞瘤有显着影响的通讯途径，全部与轴突发生和神经投射发育有关，特别是BMP7-(BMPR1B-ACVR2B)通讯途径被发现通过激活转录因子SMAD1并调节肿瘤细胞迁移。 UNK 和 MYCBP2。值得注意的是，BMP7 在有远处转移的神经母细胞瘤样本中表达较高。总之，CCPPM 提供了一种新方法来研究细胞通讯途径对疾病预后的影响，并确定了与神经发育相关的有害通讯途径。