In Figure 3, the fluorescent photos present the results of Hoechst staining on the apoptotic hallmarks induced by glutamate in cerebellar granule neurons (CGNs). Regrettably, the photo showing memantine’s attenuation were mistakenly replaced with the control group’s photos, which were used twice. In this study, memantine was included as a positive control for Hoechst staining only. The misused photos did not affect the overall conclusion of this study, as the Hoechst staining assay has been well-established. Other experiments, independent of Figure 3, have also supported the conclusions. The correct version of Figure 3 should be as follows: Figure 3. MN-12 attenuated the apoptotic hallmarks induced by glutamate in CGNs. At 8 DIV, CGNs were preincubated with or without 30 μM MN-12 or 3 μM MEM and then exposed to 100 μM glutamate 2 h later. After 24 h of glutamate challenge, CGNs were assayed with a phase contrast microscope and Hoechst 33342 staining (5 μg/mL) for 10 min and then observed by a fluorescence microscope. (A) Apoptotic cells that displayed bright blue nuclear condensations are indicated by arrows. (B) Counts of apoptotic cells by Hoechst staining as in panel A. (C) CGNs treated as mentioned above were lysed and subjected to Western blot assay by using the specific antibody against the apoptosis related protein bcl-2. Data were the mean ± SEM of three separate experiments. Scale bar 10 μM; ##p < 0.01 compared to control; **p < 0.01 compared with the glutamate group. This article has not yet been cited by other publications. Figure 3. MN-12 attenuated the apoptotic hallmarks induced by glutamate in CGNs. At 8 DIV, CGNs were preincubated with or without 30 μM MN-12 or 3 μM MEM and then exposed to 100 μM glutamate 2 h later. After 24 h of glutamate challenge, CGNs were assayed with a phase contrast microscope and Hoechst 33342 staining (5 μg/mL) for 10 min and then observed by a fluorescence microscope. (A) Apoptotic cells that displayed bright blue nuclear condensations are indicated by arrows. (B) Counts of apoptotic cells by Hoechst staining as in panel A. (C) CGNs treated as mentioned above were lysed and subjected to Western blot assay by using the specific antibody against the apoptosis related protein bcl-2. Data were the mean ± SEM of three separate experiments. Scale bar 10 μM; ##p < 0.01 compared to control; **p < 0.01 compared with the glutamate group.

中文翻译：

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对“硝酸美金刚通过体外和体内神经保护和血管舒张作用的抗痴呆药理学特征”的修正

图 3 中的荧光照片显示了小脑颗粒神经元 (CGN) 中谷氨酸诱导的细胞凋亡标志的 Hoechst 染色结果。遗憾的是，显示美金刚衰减的照片被错误地替换为对照组的照片，并使用了两次。在本研究中，美金刚仅作为赫斯特染色的阳性对照。误用的照片并不影响本研究的总体结论，因为赫斯特染色测定法已经很成熟。与图 3 无关的其他实验也支持了该结论。图 3 的正确版本应如下所示： 图 3. MN-12 减弱了 CGN 中谷氨酸诱导的细胞凋亡特征。在 8 DIV 时，CGN 在有或没有 30 μM MN-12 或 3 μM MEM 的情况下预孵育，然后在 2 小时后暴露于 100 μM 谷氨酸。谷氨酸挑战 24 小时后，用相差显微镜和 Hoechst 33342 染色 (5 μg/mL) 检测 CGN 10 分钟，然后用荧光显微镜观察。 (A) 箭头指示显示亮蓝色核浓缩的凋亡细胞。 (B) 通过 Hoechst 染色对凋亡细胞进行计数，如 A 组。(C) 将如上所述处理的 CGN 裂解，并使用抗凋亡相关蛋白 bcl-2 的特异性抗体进行蛋白质印迹测定。数据为三个独立实验的平均值±SEM。比例尺 10 μM； ##与对照相比，p < 0.01； **与谷氨酸组相比，p < 0.01。这篇文章尚未被其他出版物引用。图 3. MN-12 减弱了 CGN 中谷氨酸诱导的细胞凋亡特征。在 8 DIV 时，CGN 在有或没有 30 μM MN-12 或 3 μM MEM 的情况下预孵育，然后在 2 小时后暴露于 100 μM 谷氨酸。谷氨酸挑战 24 小时后，用相差显微镜和 Hoechst 33342 染色 (5 μg/mL) 检测 CGN 10 分钟，然后用荧光显微镜观察。 (A) 箭头指示显示亮蓝色核浓缩的凋亡细胞。 (B) 通过 Hoechst 染色对凋亡细胞进行计数，如 A 组。(C) 将如上所述处理的 CGN 裂解，并使用抗凋亡相关蛋白 bcl-2 的特异性抗体进行蛋白质印迹测定。数据为三个独立实验的平均值±SEM。比例尺 10 μM； ##与对照相比，p < 0.01； **与谷氨酸组相比，p < 0.01。