A series of 5-bromouracil derivatives were synthesized using the in situ-generated bromination method at room temperature. The synthesized compounds were evaluated in-vitro antibacterial activity against E. coli and B. cereus. In particular, compounds 2 a and 2 b exhibited prominent antibacterial activity against gram-negative E. coli with IC₅₀ values of ~9.8 and ~5.7 μg/mL. Based on the result of antibacterial studies, investigate the structure-activity relationship (SAR) and further propose the binding mode and interaction of the compounds using targets E. coli primase based on in silico molecular docking studies.

中文翻译：

---

NaBr/H2O2诱导的溴化尿嘧啶衍生物的合成及生物功能性质

采用室温原位溴化法合成了一系列5-溴尿嘧啶衍生物。评估了合成的化合物对大肠杆菌和蜡状芽孢杆菌的体外抗菌活性。特别是，化合物2a和2b对革兰氏阴性大肠杆菌表现出显着的抗菌活性，IC ₅₀值分别为~9.8和~5.7 μg/mL。基于抗菌研究的结果，利用基于计算机分子对接研究的目标大肠杆菌引物酶，研究构效关系（SAR）并进一步提出化合物的结合模式和相互作用。