The pygmy grasshoppers, which belong to the superfamily Tetrigoidea, exhibit remarkable environmental adaptability. However, no study has yet reported a reference genome for this group. In this study, we assembled a high-quality chromosome-scale genome of Eucriotettix oculatus, which survive in the environment heavily polluted by heavy metals, achieved through Illumina and PacBio sequencing, alongside chromosome conformation capture techniques. The resulting genome spans 985.45 Mb across seven chromosomes (range: 71.55 to 266.65 Mb) and features an N50 length of 123.82 Mb. Chr5 is considered to be the single sex chromosome (X). This genome is composed of 46.42% repetitive elements and contains 14,906 predicted protein-coding genes, 91.63% of which are functionally annotated. Decoding the E. oculatus genome not only promotes future studies on environmental adaptation for the pygmy grasshopper, but also provides valuable resources for in-depth investigation on phylogeny, evolution, and behavior of Orthoptera.

侏儒蝗虫属于蝗虫总科，表现出卓越的环境适应性。然而，尚未有研究报告该群体的参考基因组。在这项研究中，我们通过 Illumina 和 PacBio 测序以及染色体构象捕获技术组装了Eucriotettix oculatus的高质量染色体规模基因组，该基因组在重金属严重污染的环境中生存。所得基因组跨 7 条染色体，跨度为 985.45 Mb（范围：71.55 至 266.65 Mb），N50 长度为 123.82 Mb。 Chr5 被认为是单一性染色体 (X)。该基因组由46.42%的重复元件组成，包含14,906个预测的蛋白质编码基因，其中91.63%有功能注释。破译E. oculatus基因组不仅可以促进未来侏儒蝗虫环境适应的研究，也为深入研究直翅目昆虫的系统发育、进化和行为提供宝贵的资源。