Constructing metal–organic gels (MOGs) with enzyme-catalyzed activity and studying their catalytic mechanism are crucial for the development of novel nanozyme materials. In this study, a Co@Fe MOG with excellent peroxidase activity was developed by a simple and mild one-pot process. The results showed that the material exhibited almost a single peroxidase activity under optimal pH conditions, which allowed it to attract and oxidize the chromogenic substrate 3,3′,5,5′–tetramethylbenzidine (TMB). Based on the active electron transfer between the metal centers and the organic ligand in the synthetic material, the Co@Fe MOG–H₂O₂–TMB system was verified to be able to detect H₂O₂ and citric acid (CA). The catalytic microenvironment formed by the adsorption and the catalytic center accelerated the electron-transfer rate, which expedited the generation of hydroxyl radicals (˙OH, a kind of reactive oxygen species (ROS)) in the presence of H₂O₂. The persistence and high intensity of ˙OH generation were proven, which would endow Co@Fe MOG with a certain antibacterial ability, promoting the healing of bacteria-infected wounds. In conclusion, this study contributes to the development efforts toward the application systems of nanozymes for marker detection and antibacterial activity.

中文翻译：

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多孔网状Co@Fe金属有机凝胶：用于比色传感和抗菌应用的双功能模拟过氧化物酶纳米酶

构建具有酶催化活性的金属有机凝胶（MOG）并研究其催化机制对于开发新型纳米酶材料至关重要。在本研究中，通过简单温和的一锅法开发了具有优异过氧化物酶活性的Co@Fe MOG。结果表明，该材料在最佳pH条件下表现出几乎单一的过氧化物酶活性，使其能够吸引并氧化显色底物3,3',5,5'-四甲基联苯胺（TMB）。基于合成材料中金属中心和有机配体之间的活性电子转移，Co@Fe MOG–H ₂ O ₂ –TMB系统被验证能够检测H ₂ O ₂和柠檬酸(CA)。吸附和催化中心形成的催化微环境加速了电子转移速率，从而在H ₂ O ₂存在下加速了羟基自由基（˙OH，一种活性氧（ROS））的产生。证明了˙OH生成的持久性和高强度，这将赋予Co@Fe MOG一定的抗菌能力，促进细菌感染伤口的愈合。总之，这项研究有助于开发用于标记物检测和抗菌活性的纳米酶应用系统。