Lignosulfonates (LS) are known to reduce the recalcitrance of wood fibers to enzymatic hydrolysis. This study furthers the use of LS for the bioconversion of chemi-thermomechanical pulp (CTMP, ca. 26% lignin) at high solids content (10%) using a cellulase and hemicellulase prehydrolysis complex combined with LS loading (dual treatment). The process (10% solids, 12 h) increases the sugar yield by 20% and produces high-quality lignin-containing cellulose nanofibrils (LCNF) by mechanical fibrillation. Moreover, the dual treatment yields nanofibrils of smaller lateral size and enables better colloidal stability compared with those obtained by enzyme treatment alone or by sequential application (LS addition after enzyme). Translucent films were produced with LCNF, which are shown for their reduced hydrophilicity, high mechanical strength, and UV shielding performance. The introduced approach for efficient utilization of lignocellulose resources to coproduce high-value fibrillated lignocellulose and sugars is expected to lead to new opportunities given the availability of CTMP under the persistent reduction in demand of newsprint and printing grades.

中文翻译：

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木质素磺酸盐使得化学热机械木纤维能够同时生产原纤化木质纤维素和可发酵糖

众所周知，木质素磺酸盐 (LS) 可以降低木纤维对酶水解的抵抗力。本研究进一步利用纤维素酶和半纤维素酶预水解复合物结合 LS 负载（双重处理），将 LS 用于高固体含量 (10%) 的化学热机械纸浆（CTMP，约 26% 木质素）的生物转化。该工艺（10% 固体，12 小时）将糖产量提高了 20%，并通过机械原纤化生产出高质量的含木质素的纤维素纳米原纤维 (LCNF)。此外，与单独酶处理或顺序应用（酶后添加LS）获得的纳米纤维相比，双重处理产生的纳米纤维具有更小的横向尺寸，并且具有更好的胶体稳定性。使用 LCNF 生产半透明薄膜，其亲水性降低，机械强度高，并且具有紫外线屏蔽性能。鉴于在新闻纸和印刷级需求持续减少的情况下 CTMP 的可用性，所引入的有效利用木质纤维素资源联产高价值原纤化木质纤维素和糖的方法预计将带来新的机遇。