Integrated functionality that combines recognition elements and sensors on a single platform to provide real-time detection capabilities for fifth-generation biosensors is considered one of the dominant tools for addressing point-of-care testing in the community today. Inspired by gyroscope and smartphone-based sensors, we developed a portable and low-cost immunobiosensor for high-throughput detection of cancer-related markers. The main testing process was divided into two parts: blood sample preprocessing based on an electronic gyroscope and signal reading based on nanozyme and smartphone. The feasibility of a commercial electronic gyroscope for serum acquisition from whole blood samples was evaluated. In addition, PtCd nanoclusters (PtCd NCs) were used as excellent peroxidase-like nanozymes to trigger the target signal conversion of the immunosensor. PtCd NCs were able to catalyze the conversion of HO to ·OH, which rapidly oxidized the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) substrate to the blue Oxidized-TMB (oxTMB). Naked eye visible obvious color change and the RGB values of the color metrology system could also be read by smartphone. Prostate-specific antigen (PSA) as a model biomarker was used to validate the utility of an integrated immunoassay system to enable early cancer screening through localization of RGB values. This work focuses on convenient blood sample preprocessing and simplified serologic cancer screening steps to develop an efficient and cost-effective immunoassay platform for community and on-site point-of-care testing (POCT) in resource-limited settings.

中文翻译：

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使用电子陀螺离心机和纳米酶对癌症生物标志物进行视觉免疫生物传感的一体化智能手机成像

将识别元件和传感器结合在一个平台上的集成功能，为第五代生物传感器提供实时检测功能，被认为是当今社区中解决现场护理测试的主要工具之一。受陀螺仪和基于智能手机的传感器的启发，我们开发了一种便携式低成本免疫生物传感器，用于高通量检测癌症相关标记物。主要测试过程分为两部分：基于电子陀螺仪的血样预处理和基于纳米酶和智能手机的信号读取。评估了商用电子陀螺仪从全血样本中采集血清的可行性。此外，PtCd纳米簇（PtCd NCs）被用作优异的类过氧化物酶纳米酶来触发免疫传感器的目标信号转换。 PtCd NCs 能够催化 H2O 转化为·OH，从而快速将无色 3,3',5,5'-四甲基联苯胺 (TMB) 底物氧化为蓝色氧化 TMB (oxTMB)。肉眼可见明显的颜色变化，颜色计量系统的RGB值也可以通过智能手机读取。前列腺特异性抗原 (PSA) 作为模型生物标志物用于验证集成免疫测定系统的实用性，以通过 RGB 值的定位实现早期癌症筛查。这项工作的重点是方便的血液样本预处理和简化的血清学癌症筛查步骤，以开发一个高效且具有成本效益的免疫分析平台，用于资源有限环境中的社区和现场护理点检测 (POCT)。