Microplastics (MPs) can act as carriers of environmental arsenic species into the stomach with food and release arsenic species during digestion, which threatens human health. Herein, an integrated dynamic stomach model (DSM)-capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICPMS) is developed for online monitoring of the release and transformation behaviors of arsenic species loaded on MPs (As-MPs) in the simulated human stomach. The 3D-printed DSM with a soft stomach chamber enables the behaviors of gastric peristalsis, gastric and salivary fluid addition, pH adjustment, and gastric emptying (GE) to be controlled by a self-written program after oral ingestion of food with As-MPs. The gastric extract during digestion is introduced into the spiral channel to remove the large particulate impurity and online filtered to obtain the clarified arsenic-containing solution for subsequent speciation analysis of arsenic by CE-ICPMS. The digestion conditions and pretreatment processes of DSM are tracked and validated, and the release rates of As-MPs digested by DSM are compared with those digested by the static stomach model and DSM without GE. The release rate of inorganic arsenic on MPs is higher than that of organic arsenic throughout the gastric digestion process, and 8% of As(V) is reduced to As(III). The detection limits for As(III), DMA, MMA, and As(V) are 0.5–0.9 μg L^–1 using DSM-CE-ICPMS, along with precisions of ≤8%. This present method provides an integrated and convenient tool for evaluating the release and transformation of As-MPs during human gastric digestion and provides a reference for exploring the interactions between MPs and metals/metalloids in the human body.

中文翻译：

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动态胃模型 - 毛细管电泳 - ICPMS 用于评估消化过程中微塑料中砷形态的释放和转化行为

微塑料（MPs）可以作为环境砷物质随食物进入胃中的载体，并在消化过程中释放砷物质，威胁人类健康。本文开发了一种集成动态胃模型（DSM）-毛细管电泳-电感耦合等离子体质谱（CE-ICPMS），用于在线监测模拟人体中砷形态（As-MPs）的释放和转化行为。胃。具有柔软胃室的 3D 打印 DSM 可以在口服含 As-MP 的食物后通过自编写程序控制胃蠕动、胃液和唾液添加、pH 调节和胃排空 (GE) 的行为。将消化过程中的胃提取物引入螺旋通道去除大颗粒杂质并在线过滤，得到澄清的含砷溶液，用于后续的CE-ICPMS对砷的形态分析。对DSM的消化条件和预处理过程进行跟踪和验证，并将DSM消化的As-MPs释放率与静态胃模型和无GE的DSM消化的释放率进行比较。在整个胃消化过程中，MPs上无机砷的释放速率高于有机砷，并且8%的As(V)被还原为As(III)。使用 DSM-CE-ICPMS， As(III)、DMA、MMA 和 As(V) 的检测限为 0.5–0.9 µg L ^–1，精度为 ≤8%。该方法为评估人胃消化过程中As-MPs的释放和转化提供了一种集成且方便的工具，并为探索人体内金属/类金属与MPs之间的相互作用提供了参考。