Organic photoelectrochemical transistor (OPECT) has shown substantial potential in the development of next-generation bioanalysis yet is limited by the either-or situation between the photoelectrode types and the channel types. Inspired by the dual-photoelectrode systems, we propose a new architecture of dual-engine OPECT for enhanced signal modulation and its biosensing application. Exemplified by incorporating the CdS/Bi₂S₃ photoanode and Cu₂O photocathode within the gate-source circuit of Ag/AgCl-gated poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) channel, the device shows enhanced modulation capability and larger transconductance (g_m) against the single-photoelectrode ones. Moreover, the light irritation upon the device effectively shifts the peak value of g_m to zero gate voltage without degradation and generates larger current steps that are advantageous for the sensitive bioanalysis. Based on the as-developed dual-photoelectrode OPECT, target-mediated recycling and etching reactions are designed upon the CdS/Bi₂S₃, which could result in dual signal amplification and realize the sensitive microRNA-155 biodetection with a linear range from 1 fM to 100 pM and a lower detection limit of 0.12 fM.

中文翻译：

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双引擎增强有机光电化学晶体管以增强调制和生物分析

有机光电化学晶体管（OPECT）在下一代生物分析的发展中显示出巨大的潜力，但受到光电极类型和通道类型之间非此即彼的情况的限制。受双光电极系统的启发，我们提出了一种双引擎 OPECT 的新架构，用于增强信号调制及其生物传感应用。通过将 CdS/Bi ₂ S ₃光电阳极和 Cu ₂ O 光电阴极合并到 Ag/AgCl 门控聚（3,4-乙撑二氧噻吩）：聚（苯乙烯磺酸）（PEDOT：PSS）通道的栅源电路中，该器件与单光电极相比，表现出增强的调制能力和更大的跨导（g _{m ）。此外，器件上的光刺激有效地将}g _m的峰值移至零栅极电压而不会降低，并产生更大的电流阶跃，这有利于灵敏的生物分析。基于已开发的双光电极 OPECT，在 CdS/Bi ₂ S ₃上设计了靶介导的回收和蚀刻反应，可实现双信号放大并实现线性范围为 1 的灵敏 microRNA-155 生物检测。 fM 至 100 pM，检测下限为 0.12 fM。