Chagas disease (CD) is caused by the hemoflagellate protozoan . The control of the infection depends of the innate and acquired immune response of host. Moreover, CD plays a significant role in the immune response, and, in this context, microalgae can be an interesting alternative due to its immunomodulatory and trypanocidal effects. This study aimed to evaluate, , immunomodulatory potentials of the aqueous extracts of and . Both microalgae extracts (ME) were obtained by sonication, and the selectivity index (SI) was determined by assays of inhibitory concentration (IC) in trypomastigotes cells; as well as the cytotoxic concentrations (CC) in human peripheral mononuclear cells (PBMC). The immune response was evaluated in -infected PBMC using the IC value. ME led to inhibition of trypomastigotes after 24 h of treatment, in which the IC values were 112.1 µg/ml to and 15.8 µg ml to . On the other hand, did not affect the viability of PBMCs in concentrations up to 1000 µg ml, while was non-toxic to PBMCs in concentrations up to 253.44 µg ml. In addition, displayed a higher SI against (SI = 16.8), when compared with (SI = 8.9). decreased the levels of IFN, indicating a reduction of the inflammatory process; while displayed an interesting immunomodulatory effect, since discretely increased the levels of TNF and stimulated the production of the anti-inflammatory cytokine IL-10. This study confirms that ME are effective against trypomastigotes, and may able to control the parasitemia and preventing the progress of CD while regulating the inflammatory process.

中文翻译：

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微藻提取物调节克氏锥虫感染的人体细胞的免疫反应


恰加斯病 (CD) 是由血鞭毛虫原生动物引起的。感染的控制取决于宿主的先天性和后天性免疫反应。此外，CD 在免疫反应中发挥着重要作用，在这种情况下，微藻由于其免疫调节和杀锥虫作用可能成为一个有趣的替代品。本研究旨在评估 和 水提取物的免疫调节潜力。两种微藻提取物（ME）均通过超声处理获得，并通过锥鞭毛体细胞中的抑制浓度（IC）测定来确定选择性指数（SI）；以及人外周单核细胞 (PBMC) 中的细胞毒性浓度 (CC)。使用 IC 值评估感染的 PBMC 中的免疫反应。 ME 治疗 24 小时后对锥鞭毛体产生抑制作用，其中 IC 值为 112.1 µg/ml 至 15.8 µg/ml 至 。另一方面，浓度高达 1000 µg ml 时不会影响 PBMC 的活力，而浓度高达 253.44 µg ml 时对 PBMC 无毒。此外，与 (SI = 8.9) 相比，(SI = 16.8) 显示出更高的 SI。降低干扰素水平，表明炎症过程减少；同时表现出有趣的免疫调节作用，因为它可以离散地增加 TNF 的水平并刺激抗炎细胞因子 IL-10 的产生。这项研究证实 ME 对锥鞭毛体有效，并且可能能够控制寄生虫血症并阻止 CD 的进展，同时调节炎症过程。