The relationship between phthalates, a group of chemical pollutants classified as endocrine disruptors, and oxidative stress is not fully understood. The aim of the present hospital-based study was to explore the associations between circulating levels of 10 phthalate metabolites and 8 biomarkers of oxidative stress in adipose tissue. The study population (n = 143) was recruited in two hospitals in the province of Granada (Spain). Phthalate metabolite concentrations were analyzed by isotope diluted online-TurboFlow-LC–MS/MS in serum samples, while oxidative stress markers were measured by commercially available kits in adipose tissue collected during routine surgery. Statistical analyses were performed by MM estimators’ robust linear regression and weighted quantile sum regression. Mainly, positive associations were observed of monomethyl phthalate (MMP), monoiso-butyl phthalate (MiBP), and mono-n-butyl phthalate (MnBP) (all low molecular weight phthalates) with glutathione peroxidase (GPx) and thiobarbituric acid reactive substances (TBARS), while an inverse association was found between monoiso-nonyl phthalate (MiNP) (high molecular weight phthalate) and the same biomarkers. WQS analyses showed significant effects of the phthalate mixture on GSH (β = −30.089; p-value = 0.025) and GSSG levels (β = −19.591; p-value = 0.030). Despite the limitations inherent to the cross-sectional design, our novel study underlines the potential influence of phthalate exposure on redox homeostasis, which warrants confirmation in further research.

中文翻译：

---

西班牙成人队列中血清邻苯二甲酸盐浓度和脂肪组织氧化应激生物标志物

邻苯二甲酸盐（一组被归类为内分泌干扰物的化学污染物）与氧化应激之间的关系尚不完全清楚。本医院研究的目的是探讨脂肪组织中 10 种邻苯二甲酸酯代谢物的循环水平与 8 种氧化应激生物标志物之间的关联。研究人群（n = 143）是在格拉纳达省（西班牙）的两家医院招募的。通过在线稀释同位素-TurboFlow-LC-MS/MS 分析血清样品中的邻苯二甲酸酯代谢物浓度，同时通过市售试剂盒测量常规手术期间收集的脂肪组织中的氧化应激标记物。通过 MM 估计器的稳健线性回归和加权分位数和回归进行统计分析。主要观察到邻苯二甲酸单甲酯 (MMP)、邻苯二甲酸单异丁酯 (MiBP) 和邻苯二甲酸单正丁酯 (MnBP)（所有低分子量邻苯二甲酸酯）与谷胱甘肽过氧化物酶 (GPx) 和硫代巴比妥酸反应物质呈正相关。 TBARS），而邻苯二甲酸单异壬酯（MiNP）（高分子量邻苯二甲酸酯）与相同的生物标志物之间存在负相关。 WQS 分析显示邻苯二甲酸酯混合物对 GSH（β = -30.089；p值 = 0.025）和 GSSG 水平（β = -19.591；p值 = 0.030）有显着影响。尽管横截面设计存在固有的局限性，但我们的新研究强调了邻苯二甲酸盐暴露对氧化还原稳态的潜在影响，这需要进一步研究的证实。