The relationship between phthalate exposure and coronary heart disease (CHD) is still unclear. This study aimed to investigate the association between phthalate exposure and CHD and determine the possible atherogenic mechanisms of phthalates by assessing oxidative stress and altering miRNA expression. This case-control study included 110 participants (55 CHD patients and 55 healthy controls). The levels of oxidative stress markers, malondialdehyde (MDA), and superoxide dismutase (SOD), and the expression of miRNA-155 (miR-155) and miRNA-208a (miR-208a), were measured and correlated with the urinary mono-2-ethylhexyl phthalate (MEHP). Highly significant differences were detected between the CHD cases and the control group regarding MEHP, MDA, SOD, miR-155, and miR-208a (p-value < 0.001). Spearman correlations revealed a significant positive correlation between MDA and MEHP in urine (P = 0.001 and rs = 0.316) and a significant negative correlation between SOD and MEHP in urine (P < 0.001 and rs = −0.345). Furthermore, significant positive correlations were observed between miR-155 and urinary MEHP (P = 0.001 and rs = 0.318) and miR-208a and urinary MEHP (P < 0.001 and rs = −0.352). This study revealed an association between phthalate exposure, as indicated by urinary MEHP and CHD; altered expression of miR-155 and miR-208a and oxidative stress could be the fundamental mechanisms.

中文翻译：

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邻苯二甲酸盐暴露与冠心病：氧化应激和 miRNA 表达改变的可能影响


邻苯二甲酸盐暴露与冠心病 (CHD) 之间的关系尚不清楚。本研究旨在调查邻苯二甲酸盐暴露与 CHD 之间的关联，并通过评估氧化应激和改变 miRNA 表达来确定邻苯二甲酸盐可能的动脉粥样硬化机制。这项病例对照研究包括 110 名参与者（55 名先心病患者和 55 名健康对照）。测量氧化应激标志物丙二醛 (MDA) 和超氧化物歧化酶 (SOD) 的水平以及 miRNA-155 (miR-155) 和 miRNA-208a (miR-208a) 的表达，并与尿单邻苯二甲酸2-乙基己酯（MEHP）。 CHD 病例和对照组之间在 MEHP、MDA、SOD、miR-155 和 miR-208a 方面检测到高度显着差异（p 值 < 0.001）。 Spearman相关性显示尿液中MDA和MEHP之间呈显着正相关（P = 0.001和rs = 0.316），尿液中SOD和MEHP之间呈显着负相关（P < 0.001和rs = -0.345）。此外，miR-155 和尿液 MEHP（P = 0.001 和 rs = 0.318）以及 miR-208a 和尿液 MEHP（P < 0.001 和 rs = -0.352）之间观察到显着正相关。这项研究揭示了尿 MEHP 表明邻苯二甲酸盐暴露与冠心病之间存在关联； miR-155 和 miR-208a 表达的改变以及氧化应激可能是基本机制。