Single-cell nanoencapsulation (SCNE) has great potential in the enhancement of therapeutic effects of probiotic microbes. However, the material scope has been limited to water-soluble compounds to avoid non-biocompatible organic solvents that are harmful to living cells. In this work, the SCNE of probiotic Lactobacillus acidophilus with water-insoluble luteolin and Fe³⁺ ions is achieved by the vortex-assisted, biphasic water–oil system. The process creates L. acidophilus nanoencapsulated in the luteolin-Fe³⁺ shells that empower the cells with extrinsic properties, such as resistance to lysozyme attack, anti-ROS ability, and α-amylase-inhibition activity, as well as sustaining viability under acidic conditions. The proposed protocol, embracing water-insoluble flavonoids as shell components in SCNE, will be an advanced add-on to the chemical toolbox for the manipulation of living cells at the single-cell level.

中文翻译：

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用于增强益生菌嗜酸乳杆菌纳米封装在木犀草素-Fe3+壳中的双相水油系统

单细胞纳米胶囊（SCNE）在增强益生菌微生物治疗效果方面具有巨大潜力。然而，材料范围仅限于水溶性化合物，以避免对活细胞有害的非生物相容性有机溶剂。在这项工作中，益生菌嗜酸乳杆菌与水不溶性木犀草素和 Fe ³⁺离子的 SCNE是通过涡流辅助的双相水油系统实现的。该过程产生纳米封装在木犀草素-Fe ³⁺外壳中的嗜酸乳杆菌，赋予细胞外在特性，例如抗溶菌酶攻击、抗ROS能力和α-淀粉酶抑制活性，以及​​在酸性条件下维持活力状况。拟议的方案将水不溶性黄酮类化合物作为 SCNE 中的壳成分，将成为化学工具箱的高级附加组件，用于在单细胞水平上操纵活细胞。